Draft Genome Sequences of Two Pseudomonas Strains That Are Able To Use Furan Derivatives as Their Sole Carbon Source Carlos Farkas, a Raúl A. Donoso, a Carla Gárate-Castro, a,b Pamela Villegas, a Roberto E. Durán, c Michael Seeger, c Danilo Pérez-Pantoja a a Programa Institucional de Fomento a la Investigación, Desarrollo, e Innovación, Universidad Tecnológica Metropolitana, Santiago, Chile b Center of Applied Ecology and Sustainability (CAPES), Santiago, Chile c Laboratorio de Microbiología Molecular y Biotecnología Ambiental, Departamento de Química, Centro de Biotecnología Daniel Alkalay Lowitt, Universidad Técnica Federico Santa María, Valparaíso, Chile ABSTRACT Pseudomonas sp. strains ALS1279 and ALS1131 were isolated from wastewater treatment facilities on the basis of their ability to use furfural, a key lignocellulose-derived inhibitor, as their only carbon source. Here, we present the draft genome sequences of both strains, which can shed light on catabolic pathways for furan compounds in pseudomonads. L ignocellulosic biomass such as corn stover and wheat straw can be used in biofuel production. However, their utilization as feedstocks requires pretreat- ments that generate by-products that inhibit microbial biocatalysts (e.g., furfural and 5-hydroxymethylfurfural [HMF]) (1, 2). Consequently, biodegradative routes for lignocellulose-derived inhibitors such as furan derivatives have attracted increasing interest due to their potential to be exploited in biodetoxification strategies to remove these compounds from hydrolysates (3, 4). Here, we report the draft genome sequences of Pseudomonas sp. ALS1279 and Pseudomonas sp. ALS1131, two strains that were isolated from wastewater treatment facilities on the basis of their ability to grow with furfural as their sole carbon and energy source (5). Moreover, we tested additional catabolic capabilities, which revealed that both strains were able to use furfuryl alcohol and furoic acid as growth substrates and that ALS1279 was also able to grow with HMF, HMF acid, or HMF alcohol as its sole carbon source. Pseudomonas sp. strains ALS1279 and ALS1131 were cultured in R2A broth (Neo- gen), and genomic DNA was obtained by using the GenElute bacterial genomic DNA kit (Sigma-Aldrich), for sequencing by MicrobesNG (Birmingham, UK) using Illumina MiSeq paired-end technology (2  250 bp). Libraries with a median insert size of 504 bp (ALS1279) or 487 bp (ALS1131) were generated using the Nextera XT library preparation kit (Illumina) following the manufacturer’s protocol. A total of 641,615 reads (ALS1279) and 1,224,184 reads (ALS1131) were obtained after sequencing and trimming using Trimmomatic v0.30 (6). Reads were assembled using SPAdes v3.9 (7), and assemblies were polished with two rounds of Pilon v1.23 (8). Default parameters were used for all software programs during bioinformatic analysis. The draft genome of Pseudomonas sp. ALS1279 consisted of 183 contigs (N 50 , 63,516 bp) and was 5,309,122 bp in size, with a G+C content of 62.5% and average coverage of 50, while the genome of Pseudomo- nas sp. ALS1131 consisted of 43 contigs (N 50 , 256,635 bp) and was 5,564,837 bp in size, with a G+C content of 62.4% and average coverage of 89. Gene annotation was performed using PGAP v4.7 (9) and indicated 4,737 coding proteins for ALS1279 and 5,078 coding proteins for ALS1131. Taxonomic classification for both strains was performed, as described previously (10), by combining multilocus sequence analysis Citation Farkas C, Donoso RA, Gárate-Castro C, Villegas P, Durán RE, Seeger M, Pérez-Pantoja D. 2020. Draft genome sequences of two Pseudomonas strains that are able to use furan derivatives as their sole carbon source. Microbiol Resour Announc 9:e01131-19. https://doi.org/10.1128/MRA.01131-19. Editor Vincent Bruno, University of Maryland School of Medicine Copyright © 2020 Farkas et al. This is an open- access article distributed under the terms of the Creative Commons Attribution 4.0 International license. Address correspondence to Danilo Pérez- Pantoja, danilo.perez@utem.cl. Received 26 September 2019 Accepted 6 December 2019 Published 9 January 2020 GENOME SEQUENCES crossm Volume 9 Issue 2 e01131-19 mra.asm.org 1 on July 24, 2020 by guest http://mra.asm.org/ Downloaded from