Research Article Potential Effect of Leukocyte-Platelet-Rich Fibrin in Bone Healing of Skull Base: A Pilot Study Felipe Fredes, 1 Jaime Pinto, 2 Nelson Pinto, 3 Pablo Rojas, 1 Daniel M. Prevedello, 4,5 Ricardo L. Carrau, 4,5 and Thomas Schmidt 1 1 Department of Otolaryngology-Head & Neck Surgery, Universidad de Concepci´ on, Concepci´ on, Chile 2 Department of Neurosurgery, Universidad de Concepci´ on, Concepci´ on, Chile 3 Graduate School of Periodontics and Implant Dentistry, University of the Andes, Research Center for Regenerative Medicine and Tissue Engineering, Concepci´ on, Chile 4 Department of Otolaryngology-Head & Neck Surgery, Te Ohio State University Wexner Medical Center, Columbus, OH 43210, USA 5 Department of Neurologic Surgery, Te Ohio State University Wexner Medical Center, Columbus, OH 43210, USA Correspondence should be addressed to Tomas Schmidt; emsaschmidt@gmail.com Received 28 January 2017; Revised 4 September 2017; Accepted 11 October 2017; Published 16 November 2017 Academic Editor: Cherie-Ann Olympia Nathan Copyright © 2017 Felipe Fredes et al. Tis is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Background. Reconstruction of surgical defects following cranial base surgery is challenging. Others have demonstrated that leukocyte-platelet-rich fbrin (L-PRF) stimulates tissue healing and bone regeneration. However, these studies have addressed mostly maxillofacial surgical wounds. Objective. Te objective of this study was to assess the possible adjuvant role of L-PRF in inducing neoossifcation of the surgical bone defect in anterior skull base surgery. Methods. We identifed patients who had undergone an endoscopic endonasal surgery of the anterior skull base in which L-PRF membranes were used for the reconstruction of the bone defect and who were followed up with postoperative CT scans. CT fndings were then correlated with baseline scans and with the CT scans of a patient who had undergone imaging and histologic analysis afer maxillofacial surgery in which L-PRF was used and in which we demonstrated bone formation. Results. Five patients fulflled the inclusion criteria. In four patients, the CT scan demonstrated closure of the bony defect by neoosteogenesis; however, the bone appeared less dense than the surrounding normal bone. A comparison with the control patient yielded similar radiological features. Conclusion. Tis case series suggests that L-PRF may induce bone healing and regeneration at the surgical site defect. Multi-institutional studies with a larger series of patients are required to confrm this possibility. 1. Introduction Te endonasal endoscopic approach for lesions of the anterior skull base has decreased the morbidity associated with open approaches while achieving similar results in terms of disease control. Te use of vascularized tissues to repair surgical defects has dramatically decreased the rate of postoperative complications such as cerebrospinal fuid (CSF) leaks and infections [1]. Te introduction of the nasoseptal fap in 2006 reduced the incidence of CSF leaks from 30% to 5% [2]. Despite these advances in reconstructive skull base surgery, complications continue to be reported, so the search for new therapeutic alternatives is necessary to further reduce morbidity. Te use of platelet concentrates in surgery and wound management is an attractive option to enhance tissue healing. One of these products is the leucocyte and platelet-rich fbrin (L-PRF), a platelet concentrate characterized by containing a dense matrix of fbrin, platelets, and leucocytes, thus representing a considerable improvement over its prede- cessor, platelet-rich plasma (PRP) [3]. PRP products are preparations without leukocytes and with a low-density fbrin network, and all the products of this family can be used as liquid solutions or in an activated gel form, but not as a solid flling material like L-PRF [3]. L-PRF is a simple, natural, and inexpensive product obtained from the patient’s blood, which is centrifuged without using anticoagulants. Coagulation occurs during centrifugation, leading to the Hindawi International Journal of Otolaryngology Volume 2017, Article ID 1231870, 7 pages https://doi.org/10.1155/2017/1231870