Research Article Soft Tissue Augmentation with Autologous Platelet Gel and -TCP: A Histologic and Histometric Study in Mice Antonio Scarano, 1 Maurizio Ceccarelli, 2 Massimiliano Marchetti, 3 Adriano Piattelli, 2 and Carmen Mortellaro 4 1 Department of Medical, Oral and Biotechnological Sciences and CeSI-MeT, University of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy 2 Department of Medical, Oral and Biotechnological Sciences, University of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy 3 Department of Surgery Sciences, University of Rome “La Sapienza”, Piazzale Aldo Moro 5, 00185 Roma, Italy 4 Oral Surgery Unit, Regina Margherita Pediatric Hospital and University of Eastern Piedmont, Viale Piazza d’Armi 1, 28100 Novara, Italy Correspondence should be addressed to Antonio Scarano; ascarano@unich.it Received 17 March 2016; Revised 12 May 2016; Accepted 14 June 2016 Academic Editor: Homayoun H. Zadeh Copyright © 2016 Antonio Scarano et al. Tis is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Background. Facial aging is a dynamic process involving both sof tissue and bony structures. Skin atrophy, with loss of tone, elasticity, and distribution of facial fat, coupled with gravity and muscle activity, leads to wrinkling and folds. Purpose. Te aim of the study was to evaluate microporous tricalcium phosphate (-TCP) and autologous platelet gel (APG) mix in mice for oral and maxillofacial sof tissue augmentation. Te hypothesis was that -TCP added with APG was able to increase the biostimulating efect on fbroblasts and quicken resorption. Materials and Methods. Ten female, 6–8-week-old black-haired mice were selected. - TCP/APG gel was injected into one cheek; the other was used as control. Te animals were sacrifced at 8 weeks and histologically evaluated. Results. Te new fbroblast was intensively stained with acid fuchsin and presented in contact with -TCP. At higher magnifcation, actively secreting fbroblasts were observed at the periphery of -TCP with a well diferentiated fbroblast cell line and blood vessels. Acid fuchsin stained cutaneous structures in pink: no epidermal/dermal alterations or pathological infammatory infltrates were detected. Te margins of -TCP granules were clear and not difused near tissues. Conclusion. APG with -TCP preserves skin morphology, without immune response, with an excellent tolerability and is a promising scafold for cells and biomaterial for sof tissue augmentation. 1. Introduction Facial aging is characterized by skin changes, sagging, and volume loss. Vashi et al. detected that this process begins with surface and subsurface structural changes in multiple facial tissue layers, including skin, fat, muscle, and bone [1]. Facial tissue layers age interdependently, contributing to the overall facial appearance. As discussed by Richard et al. skeletal bone is a scafold for the sof tissue [2]. Facial aging is a dynamic process involving the aging of sof tissue and bony structures. Sjerobabski-Masnec and ˇ Situm reported that a multisystem degenerative process involves the skin and the skin support systems including the bone, cartilage, and subcutaneous compartments [3]. Recent evidence from Mendelson et al. clearly demon- strates that the aging of the face is primarily one of bone resorption [4] and changes in bones and the facial skeleton have a signifcant efect [5]. So, as seen by Levine et al., any kind of changes in the mandibular and maxilla projection, width or height, can afect overall aesthetics [6]. Te midface skeleton is formed by the maxilla in the medial and middle thirds and, as highlighted by Soares and Silver, it is completed by the body and arch of the zygoma in the lateral third [7]. Te maxillary angle (superior-to-inferior maxilla at the Hindawi Publishing Corporation BioMed Research International Volume 2016, Article ID 2078104, 7 pages http://dx.doi.org/10.1155/2016/2078104