Influence of Electroporation on HT29 Cell Proliferation, Spreading and Adhesion Properties Hassan Buhari Mamman, Muhammad Mahadi Abdul Jamil, Nur Adilah Abd Rahman, Radzi Ambar, and Chew Chang Choon Abstract The aim of this study is to investigate the influence of pulse electric field on cell proliferation, spreading and adhesion properties of HT29 cell line towards the enhancement of tissue regeneration and wound healing process. The HT29 cells were treated with an electric field of 600 V/cm for 500 μs in vitro. A time-lapse live imaging of the adhesion properties of the HT29 cell was carried out using integrated devices that was equipped with digital camera and an inverted microscope. The study found out that when HT29 cell lines were electroporated with 600 V/cm and 500 μs pulse duration, it reached 96.1% confluence after 64 h of seeding whereas the non-electroporated (NEP) cells reached only 76% confluence after 64 h. Interestingly, both the EP cell and the NEP cell attained their maximum length of 34.76 and 29.73 μm respectively after 24 h of seeding. Furthermore, the electric treatment was found to decrease the adhesiveness of the cell where it detaches from the substrate after 5.6 min as compared to control group that took 8 min to com- pletely detach from substrate. Hence, the study suggests that the application of appropriate electric filed treatment, can cause cellular changes in cells such as proliferation and adhesion which could contribute to the facilitation of wound healing process via increased cellular proliferation and migration. Keywords Adhesion Á Electroporation Á Proliferation Á Cell size Á Wound healing 1 Introduction Adhesion of cells to each other and to their ECM is important in creating cell shape and organization in tissue engineering. Likewise, comprehending how cells adhere is significant in knowing disease development like cancer and muscular dystrophies which mainly comprise of failure in cell adhesion. Additionally, cell adhesion and H. B. Mamman Á M. M. Abdul Jamil (&) Á N. A. Abd Rahman Á R. Ambar Á C. Chang Choon Biomedical Modelling and Simulation Research Group, Faculty of Electrical and Electronics Engineering, Universiti Tun Hussein Onn Malaysia, Batu Pahat, Johor, Malaysia e-mail: drmuhammadmahadi@gmail.com © Springer Nature Singapore Pte Ltd. 2021 Z. Md Zain et al. (eds.), Proceedings of the 11th National Technical Seminar on Unmanned System Technology 2019, Lecture Notes in Electrical Engineering 666, https://doi.org/10.1007/978-981-15-5281-6_55 761