Hassan and Mujtaba, IJPSR, 2017; Vol. 8(7): 3151-3154. E-ISSN: 0975-8232; P-ISSN: 2320-5148 International Journal of Pharmaceutical Sciences and Research 3151 IJPSR (2017), Volume 8, Issue 7 (Research Article) Received on 18 January, 2017; received in revised form, 13 June, 2017; accepted, 21 June, 2017; published 01 July, 2017 ORAL NANO-EMULSION OF FENUGREEK OIL FOR TREATMENT OF DIABETES Khidir A. M. Hassan * and Md Ali Mujtaba Pharmaceutics Department, Faculty of Pharmacy, Northern Border University, Kingdom of Saudi Arabia. ABSTRACT: Diabetes mellitus (DM) is one of the principal causes of death in the world. DM causes many diseases with different pathogenesis disturbing glucose, protein and lipid metabolism. The primary characteristic is elevated glucose, blood level, which may be due to deficiency in the secretion of insulin and / act. Fenugreek oil is covered to recover glucose levels, intolerance, and insulin sensitivity as compared to the diabetic group. It is claimed that there is less damage of β-cell of pancreatic islet after dosing of fenugreek oil in diabetic rats. The objective of the study was to formulate a nano formulation of fenugreek oil, so as to enhance the effectiveness of fenugreek oil in treating induced diabetes by intraperitoneal (IP) injection of streptozocin (45 mg / kg) in Wistar albino rats, since nano formulation is claimed to enhance the bioavailability of bioactive compounds. A nano- emulsion was developed by adopting emulsion phase inversion and emulsion titration technique. The nano-emulsion with droplet size with less than 100 nm in diameter was stabilized by non –ionic surfactants. Ternary systems of three components made up fenugreek oil, Tween 80 and distilled water were set up at different weight ratios and mixed via vortex agitation. From this study it was clear that the developed nano formulation has considerably reduced the glucose level in blood in the diabetic rats, whereas there is no reduction in cholesterol, HDL cholesterol, blood level in diabetic rats, whereas a significant reduction in LDL cholesterol level is realized. INTRODUCTION: DM is highly dominant and has potential detrimental effects on a patient's health, which can result in a complicated condition is a major health concern 1 . DM is one of the major cases of demise in the world 2 . DM is a made up of various disorders with different pathogenesis that affect glucose, protein and lipid metabolism. The primary characteristic is elevated glucose level that results from either deficiency in insulin secretion, and / or act. QUICK RESPONSE CODE DOI: 10.13040/IJPSR.0975-8232.8(7).3151-54 Article can be accessed online on: www.ijpsr.com DOI link: http://dx.doi.org/10.13040/IJPSR.0975-8232.8 (7). 3151-54 Numerous pathogenic courses are taken in the development of diabetes e.g. autoimmune destruction of the β-cells of the pancreas, abnormalities that lead to insulin resistance. In reference to damaging properties of the chemical synthesized medicines, incapability of current medicines to control features of DM disorders, the high medicines cost, other strategies for present pharmacotherapy of DM is immediately needed. The utilization of herbal medications will be the alternative medicines; as those herbal medicines are showing less toxicity, harmless and are with minimum side effects 3 . Trigonella foenum-graecum (fenugreek), has varied biological actions and pharmacological functions. The seeds are employed customary to control DM 4 . Keywords: Diabetes mellitus, Insulin, Streptozocin, Nano- formulation, Fenugreek oil Correspondence to Author: Dr. Khidir A. M. Hassan Assistant Professor, Pharmaceutics Department, Faculty of Pharmacy, Northern Border University, Kingdom of Saudi Arabia. E-mail: khidiragab@gmail.com