Thapliyal et al (2013) Biotechnology International 6(4): 48-57 48 ©Biotechnology Society www.bti.org.in ISSN 0974-1453 Research Article QUERCETIN, BERGAPTEN AND BARBERINEB AS ANALOGUES OF RIFAMPICIN AND ISONIAZID SCREENED IN-SILICO FROM HERBAL PLANTS OF UTTARAKHAND FOR THE TREATMENT OF TUBERCULOSIS (TB) Taranjeet Kapoor, Prabhakar Semwal, Prashant Anthwal, Madhu Thapliyal 1 , AshishThapliyal* Department of Biotechnology, Graphic Era University, Dehradun (UK), India-248002 *Corresponding author: ashish.thapliyal@gmail.com 1 Dept. of Zoology, Pt. LMS Govt. PG College, Rishikesh, India ABSTRACT: Mycobacterium tuberculosis (MBT) has become resistant to most of the drugs that were being used for the treatment of tuberculosis (TB). With this increase in the resistance of MBT towards almost all drugs, the need for new drugs that can inhibit the activity of MBT and treat millions of patients worldwide is one of the major reasons of concern. In-silico analysis of phytochemicals has provided the potential to screen numerous phytochemicals. By employing a Molecular docking software iGEMDOCK, 3 possible analogues of Rifampicin and Isoniazid which are known inhibitors of MBT were screened based on the comparison of their binding energies to the known inhibitors. Isoniazid inhibits Enoyl-acyl carrier protein (InhA), which is responsible for the synthesis of type II fatty acid and Rifampicin inhibits the protein mycobacterium dependent RNA- polymerase required for RNA synthesis. Quercetin, Bergapten and Barberineb are the chemical compounds which can be the best possible analogues of Rifampicin and Isoniazid as they bind to their target proteins with the same binding energy as the two known inhibitors. Keywords: Mycobacterium tuberculosis (MBT), iGEMDOCK, Rifampicin, Isoniazid, Quercetin, Bergapten and Barberineb.