Free Radical Scavenging Activity of Aerial Parts of Artemisia pallens in Mediating Hepatoprotective Activity of RIF+INH Intoxicated Rats Praveen Kumar Ashok 1,a* , Poonam Rishishwar 2 , Kumud Upadhyaya 3 1 Faculty of Pharmacy, GRD (PG) IMT, Dehradun.248009 2 Faculty of Pharmaceutical Sciences Shri Venkateshwara University Gajraula, Uttar Pradesh 3 Faculty of Pharmacy, Kumaun University, Bhimtal, Nanital.243136 pkashok157@gmail.com a* , Tel.: +918279392039 Keywords: Artemisia pallens, free radical, hepatoprotective, SGOT, SGPT. Abstract. To investigate the free radical scavenging & hepatoprotective activity of phenolic rich fraction of Artemisia pallens on RIF+INH induced oxidative stress in Sprague dawley rats. Free radical scavenging and hepatoprotective activity was evaluated by using DPPH, Nitric oxide, Superoxide radical and Hydroxyl radical assay models and induced RIF+INH intoxicated rats. The total phenolic content was found to be 312.60 µg gallic acid equivalents (GAE)/gm of dry extract. The total flavonoid content was found to be 322.20 µg rutin equivalents (RUE)/gm of dry extract. In the current study, free radical scavenging activity was evaluated by using 1,1-diphenyl-2- picrylhydrazyl (DPPH), nitric oxide, superoxide radical and hydroxyl radical scavenging activity were found to be (42.25 ± 0.95) and (09.16 ± 1.62) µg/ml, (101.62 ± 1.64) and (32.41 ± 1.24) µg/ml, (72.62 ± 1.86) and (10.28 ± 1.96) µg/ml, (33.82 ± 1.12) and (12.82 ± 1.86) µg/ml, respectively. There was also a dose dependent increase in reductive ability of Artemisia pallens extract with increase in concentration and were further investigated in invivo hepatoprotective activity experiment against toxicity induced by RIF+INH. The free radical scavenging and hepatoprotective activity may be attributed to the presence of phenolic compounds and histology of the liver section of the animals treated with the extracts showed the presence of normal hepatic cords, absence of necrosis and fatty infiltration, which further evidenced the hepatoprotective activity of Artemisia pallens. Introduction Liver cells possess variety of compensative mechanisms to deal with reactive oxygen species (ROS) and their effect; among these are the induction of variety of antioxidant proteins comparable to superoxide dismutase (SOD), catalose, gluthathione peroxidase (GSHPx) and therefore the tripeptide glutathione (GSH). Therefore, oxidative stress, caused primarily by ROS, is additionally related to hepatic diseases[1]. Tuberculosis (TB) may be a unsafe disease, that gradually swallows the life human being. The world prevalence of T.B. was thirty second (1.86 billion people) and therefore the international case mortality was twenty third[2]. Dietary antioxidant intake is also a very important strategy for inhibiting or delaying the oxidisation of susceptible cellular substrates, and is therefore relevant to disease prevention in several paradigms. phenolic compounds like flavonoids, phenolic acids, diterpenes and tannins have received attention for their high antioxidative activity[3]. Oxidative damage caused by free polygenic disorder, cancer and liver disease[4]. Antioxidant compounds reduce the action of reactive oxygen species (ROS) in damaged tissues throughout the recovery method[5]. In view of severe undesirable side result of synthetic agents, there's growing focus to follow systematic research methodology and to evaluate scientific basis for the normal herbal medicines that are claimed to possess hepatoprotective activity. The utilization of medicinal plants in modern drugs suffers from the very fact that although many plants are utilized in the world to prevent or to cure diseases, scientific evidence in terms of recent medication is lacking in most cases. But nowadays it's necessary to provide scientific proof as whether or not to justify the utilization of plant or its active principles[6]. International Journal of Pharmacology, Phytochemistry and Ethnomedicine Submitted: 2019-01-07 ISSN: 2297-6922, Vol. 14, pp 1-15 Revised: 2019-08-26 doi:10.18052/www.scipress.com/IJPPE.14.1 Accepted: 2019-11-19 2019 SciPress Ltd, Switzerland Online: 2019-11-28 SciPress applies the CC-BY 4.0 license to works we publish: https://creativecommons.org/licenses/by/4.0/