1 Neuroligin-2 dependent conformational activation of Collybistin reconstituted in supported hybrid membranes Jonas Schäfer 1 , Lucas Förster 1 , Ingo Mey 1 , Theofilos Papadopoulos 2 , Nils Brose 3* , Claudia Steinem 1,4* 1 Institute for Organic and Biomolecular Chemistry, Georg August University, Göttingen, Germany 2 Department of Molecular Biology, University Medical Center, Göttingen, Germany 3 Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, Göttingen, Germany 4 Max Planck Institute for Dynamics and Self-Organization, Göttingen, Germany *Corresponding authors: Claudia Steinem E-mail: csteine@gwdg.de Nils Brose E-mail: brose@em.mpg.de Running title: Activation of collybistin by neuroligin 2 Keywords: GABAA receptor organisation, inhibitory postsynapse, phosphoinositides (PtdInsPs), reflec- tometric interference spectroscopy (RIfS), supported hybrid membranes (SHMs), https://www.jbc.org/cgi/doi/10.1074/jbc.RA120.015347 The latest version is at JBC Papers in Press. Published on October 30, 2020 as Manuscript RA120.015347 by guest on December 12, 2020 http://www.jbc.org/ Downloaded from