International Journal of Health Sciences and Research Vol.10; Issue: 1; January 2020 Website: www.ijhsr.org Original Research Article ISSN: 2249-9571 International Journal of Health Sciences and Research (www.ijhsr.org) 201 Vol.10; Issue: 1; January 2020 The Effect of Intermittent Negative Pressure on the Amputation Stump of Adult Mice: A Pilot Study Osama Sharafeldin Mohammedahmed Abbadi 1 , Khalid Mohamed Nafie 2 1 Lecturer of Biochemistry, Faculty of Medicine, Omdurman Islamic University, Sudan. 2 Consultant of Pathology and Laboratory Medicine, Ministry of Health, Saudi Arabia, Prince Mishari bin Saud Hospital, Saudi Arabia. Corresponding Author: Osama Sharafeldin Mohammedahmed Abbadi ABSTRACT Overview: Limb amputation could occur accidently or due to an inevitable medical indication such as cancer, vascular damage, or traumatic destruction. Whatever the cause, a lost limb does not regenerate in mammals and most animals; however, studies showed that young mice have an ability to restore a distally amputated finger tip. There are evidences the in literature supporting that applying intermittent negative pressure to tissues could trigger the release of growth factors; which are important for tissue regeneration. Objectives: To document the effects of the intermittent negative pressure-INP in an amputation stump of albino mice, particularly if there are evidence of cellular proliferation or tissue remodeling. Methods: Two albino mice, three weeks of age, were first cut at the wrist level on their right arms. One mouse was subjected to manual INP by a fifty milliliter pump syringe, ten minutes daily for three weeks, the other mouse left to heal spontaneously, as a control. Results: There was an excessive fibrous tissue formation within the stump, seen by the naked eye. Microscopic examination showed thinning of the epithelial lining, areas of fluid and inflammatory cells collection, and excessive fibrous tissue formation. Conclusion: Negative pressure does not enhance the growth or regeneration of amputated limbs in adult mice; instead, it caused an inflammatory-like response and fibrous tissue formation. Keywords: Amputation, Negative pressure, Albino mice. INTRODUCTION Amputation is the act of removal- especially by cutting- of part of the body. The term is used mainly to the limbs, but it could include other body parts such as the nose, ears, tongue, penis, and sometimes for the breasts. Among all species, amphibians have a distinctive ability to reform lost parts of their body, [1,2] and this might be due to the late onset of maturation of limb buds, which make their development to be independent from other embryonic roots. [1] Other amniotes cannot regenerate a limb, but mice embryos shown to restore only the tip of the digit. [3,4] The real difference between the amphibians and other organisms that they retain their abilities to produce Blastema cells; cells that can grow and differentiate to different cell lineages. [4] Other animals usually loose theses cell clusters after birth. [3,4] The limb regenerative capacity of mice is very limited, as for all mammals, [4] and the ability of the fingertip of mice to regenerate is explained by its pattern of ossification: fingertips ossify at the very distal tip of bone surface, while the other proximal bony tissues ossifies and gain length through the embryonic end plates. [5] The use of negative pressure had become a cornerstone in the treatment of chronic wounds. The negative pressure wound therapy (NPWT) is a mechanical system uses the application of negative