IOSR Journal of Applied Chemistry (IOSR-JAC) e-ISSN: 2278-5736.Volume 7, Issue 7 Ver. I. (July. 2014), PP 62-67 www.iosrjournals.org www.iosrjournals.org 62 | Page Analysis of 5-Hydroxymethyl-2-Furfuraldehyde in Electrolytic Products and Energy Drink Products Pornpimol Muangthai 1 , Yonrawee Wiwatchankit 1 , 1 Department of Chemistry, Faculty of Science, Srinakharinwirot University,Bangkok,Thailand Abstract: Electrolytic products and energy drink products are used as electrolyte to heal diarrehoea, energy serving for athlete and labour people.Both products contain the main component such as sodium ion, potassium ion ,sugar, artificial flavor and colour. 5-Hydroxymethyl-2-furfuraldehyde(HMF) is the one product from browning reaction in food. It was claimed as a toxic substance. The aim of this work was to analyse HMF content in electrolytic products and energy drink products by High Performance Liquid Chromatography. The optimum conditions for analysis HMF were studied and validated method, then applied for analysis HMF content in products. The optimization method showed that HMF could be eluted with isocratic elution by the mixture of water/acetonitrile (90:10, v/v) that controlled flow rate at 1.5 ml/min. and detected at 280 nm by diode array detector (DAD). The limit of detection (LOD) and limit of quantitation (LOQ) were 0.89 µg/L and 2.9 µg/L, respectively. The relative standard deviation(%RSD) ( replicate numbers = 10) was 0.55%.The HMF could be detected in both samples products within the range of 0.03- 6 mg/L.This work proved that the toxic substance such as hydroxymethylfurfuraldehyde present in the electrolytic products and energy drink products, so the consumer should care about the using of electrolytic products. Keywords: 5-Hydroxymethyl-2-Furfuraldehyde,HMF , Electrolytic Products, Energy Drink Products I. Introduction Electrolyte products are the healthy products used for medical purpose such as the deficiency of electrolyte in human body.An important electrolyte products as saline or normal saline frequently used for patients who cannot take fluids orally by intravenous drips[1]. Normal saline is also used for aseptic purpose , used for nasal washes to relieve the symptoms of the cold too[2].Other saline products as ophthalmic eye drops are the saline – containing drops used as an ocular route to administer that may contained steroids, antihistamines, sympathomimetics, non-steroidal anti-inflammatory drugs or topical anesthetics [2]. Hypertonic saline are mucoactive agent used to dissolve the mucous in throat or internal tract of nose. However, some of the electrolyte products are formulated for sold as powder oral rehydration salts form. This product is very popular for diarrehoea people to heal the electrolytic balance in body. All above referred electrolyte products are the electrolyte in the form of saline and oral rehydration electrolyte , which contain sodium chloride in the range of 0.22 – 7% and dextrose or glucose in the range of 4 -5 %[3]. Energy drink products is now the one popular products among the youth people[4,5].The strategies to sale the energy drink also state that the energy drink products perform energy for athletic performance[6]. The youth people consume energy drinks products prior to or during exercise and sporting events to boost their physical performance[7,8]. Now,energy drink products is produced by mixing with many substance such as food color , food flavour , high sugar content and caffeine[9]. There have many reports inform some toxic from drinking the energy drink products such as cardiac dysrhythmias , dilated cardiomyopathy, rhabdomyolysis,kidney failure and sudden death[10,11]. However, there has no report about the content of the browning substance as 5-Hydroxymethyl-2- furfuraldehyde. 5-Hydroxymethyl-2- furfuraldehyde(HMF) is an important products from nonenzymatic browning reaction and forms by the reaction between carbonyl group in sugar and amino group in amino acid of protein which is known as Maillard browning reaction.[12,13]. It has a formular structure as showed in fig. 1. Figure1.Structural formula of 5-Hydroxymethyl- 2-furfuraldehyde