BIODIVERSITAS ISSN: 1412-033X Volume 22, Number 11, November 2021 E-ISSN: 2085-4722 Pages: 4716-4721 DOI: 10.13057/biodiv/d221102 Phytochemical screening and antioxidant activity of wild mushrooms growing in tropical regions ELISA HERAWATI 1,2 , RICO RAMADHAN 3,6 , FARIDA ARIYANI 1 , MARJENAH 2,4 , IRAWAN WIJAYA KUSUMA 2,4,5 , WIWIN SUWINARTI 2,4 , DJUMALI MARDJI 2,4 , RUDIANTO AMIRTA 2,4 , ENOS TANGKE ARUNG 2,4,5,♥ 1 Politeknik Pertanian Negeri Samarinda. Jl. Samratulangi, Sei Keledang, Samarinda 75242, East Kalimantan, Indonesia 2 Graduate School of Forestry, Universitas Mulawarman. Jl. Penajam, Gunung Kelua, Samarinda 75123, East Kalimantan, Indonesia 3 Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga. Jl. Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia 4 Faculty of Forestry, Universitas Mulawarman. Jl. Penajam, Gunung Kelua, Samarinda 75123, East Kalimantan, Indonesia. Tel.: +62-541-749068, Fax.: +62-541-735379,  email: tangkearung@yahoo.com 5 Research Center for Drugs and Cosmetics from Tropical Rainforest Resources, Universitas Mulawarman. Jl. Ki Hajar Dewantara, Gunung Kelua, Samarinda 75123, East Kalimantan, Indonesia 6 Division of Exploration and Synthesis of Bioactive Compounds, Research Center for Bio-Molecule Engineering, Universitas Airlangga. Jl. Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia Manuscript received: 23 June 2021. Revision accepted: 6 October 2021. Abstract. Herawati E, Ramadhan R, Ariyani F, Marjenah, Kusuma IW, Suwinarti W, Mardji D, Amirta R, Arung ET. 2021. Phytochemical screening and antioxidant activity of wild mushrooms growing in tropical regions. Biodiversitas 22: 4716-4721. This study aims to determine the presence of secondary metabolites which generally have bioactivity that is antioxidant with phytochemical tests and antioxidant tests in Auricularia auricula, Schyzophyllum commune, Microporus xanthopus, and Trametes versicolor fungi that grow in the wild. Phytochemical analysis was carried out by testing color changes and modifications, while antioxidant tests were carried out by using a UV-Vis spectrophotometer and a DPPH solution (1,1-diphenyl-2-picrylhydrazyl). This research found that edible wood fungus that grows, wild such as A. auricula, contains secondary metabolites, namely flavonoids, triterpenoids, saponins, and tannins, but does not contain steroids, alkaloids, carotenoids, or coumarin, while S. commune contains secondary metabolites, namely flavonoids, steroids, tannins, and coumarin, but does not contain triterpenoids, alkaloids, carotenoids, or saponins and non-edible wood fungus, such as M. xanthopus, contains secondary metabolites, namely, flavonoids, saponins, tannins, coumarin, and steroids, but does not contain triterpenoids, alkaloids, or carotenoids, while T. versicolor contains compounds, secondary metabolites, flavonoids, saponin, tannins, coumarin, and triterpenoids, but does not contain steroids, alkaloids, or carotenoids. The antioxidant test results are based on IC50 calculations; it is known that the IC50 value of A. auricula is 499.25 g/mL, while S. commune is 121.37 g/mL, M. xanthopus is 251.20 g/mL, and T. versicolor is 493.04 g/mL. Based on the research results, edible and inedible wood mushroom extracts contain several phytochemicals and show potential for antioxidant activity. Keywords: Auricularia auricula, Microporus xanthopus, Schyzophyllum commune, Trametes versicolor, wood fungus INTRODUCTION Wood-rot fungi are wild fungi in nature that grow and live on logs with varying degrees of weathering, e.g. Microporus xanthopus, Auricularia auricula, Trametes versicolor, and Schyzophyllum commune. There are wood- rot fungi that can be consumed, such as A. auricula and S. commune, while non-edible ones, such as M. xanthopus and T. versicolor, are a group of fungi that can break down cellulose and lignin so that wood becomes rotten, and the strength of its elastic fibers drops rapidly (Herliyana et al. 2011; Julian et al. 2019). Fungi or mushrooms are plants that are heterotrophic and do not contain chlorophyll, to meet their daily needs they take ready-made food substances from other organisms. These fungi require a food source as a substrate, an energy source (from the oxidation of carbon compounds), metabolic activity (for the growth and development of hyphae), and nutrients (Arbaain et al. 2019). Mushrooms contain various secondary metabolite compounds that contribute to various biological activity, apart from being a nutrient-rich food source and non-toxic drugs that have physiological benefits (Mirfat et al. 2010). Metabolite compounds in plants are classified into two categories, namely, primary metabolites and secondary metabolites. Primary metabolites are formed limited quantities and are used for the growth and life of organisms (Nofiani 2008). Secondary metabolites are compounds produced by organisms as protection against extreme environmental conditions or from predatory threats. Secondary metabolites are not used for growth but are transformed into primary metabolites under stressful conditions (Nofiani 2008). Secondary metabolite compounds are chemical compounds that generally have bioactivity ability to and function as protectors of these plants from pests and diseases, for the plant itself or its environment. The benefits of wood-rot fungus secondary metabolites have been reported by several researchers, including the secondary metabolite compounds of Auricularia auricula fungi that are anti-fungal (Triani et al. 2017), and potentially an anti-microbial herbal treatment for bacterial and fungal pathogens in humans (Oli et al. 2020), they also