~ 48 ~ International Journal of Herbal Medicine 2018; 6(4): 48-51 E-ISSN: 2321-2187 P-ISSN: 2394-0514 IJHM 2018; 6(4): 48-51 Received: 12-05-2018 Accepted: 16-06-2018 Malkhaz Getia TSMU I. Kutateladze Institute of Pharmacochemistry, Tbilisi, Georgia Vakhtang Mshvildadze TSMU I. Kutateladze Institute of Pharmacochemistry, Tbilisi, Georgia, University of Quebec at Chicoutimi, LASEVE, G7H 2B1, Chicoutimi, Canada Nino Tabatadze TSMU I. Kutateladze Institute of Pharmacochemistry, Tbilisi, Georgia Genri Dekanosidze TSMU I. Kutateladze Institute of Pharmacochemistry, Tbilisi, Georgia Jean Legault University of Quebec at Chicoutimi, LASEVE, G7H 2B1, Chicoutimi, Canada Andre Pichette University of Quebec at Chicoutimi, LASEVE, G7H 2B1, Chicoutimi, Canada Correspondence Malkhaz Getia TSMU I. Kutateladze Institute of Pharmacochemistry, Tbilisi, Georgia Biological active compounds from Betula megrelica grown in georgia Malkhaz Getia, Vakhtang Mshvildadze, Nino Tabatadze, Genri Dekanosidze, Jean Legault and Andre Pichette Abstract Phytochemical investigation of the bark of Betula megrelica Sosn. grown in Georgia led to the isolation and identification of eleven biologically active compounds from ethanol extract. Their structures were determined by NMR, MS, UV and IR methods. The crude extract and enriched fractions from the bark of B. megrelica, were studied in vitro experiments to reveal their anti-inflammatory, anti-oxidant and anti- cancer activities. Keywords: Betula megrelica, spectral identification, phenylethanoid 1. Introduction Gen. Betula L. (Betulaceae) in Georgian flora is presented with 7 varieties. Among of them, Betula megrelica Sosn. (Figure 1.) Is an endemic species [1] . Gen. Betula L. is known for its rich constituent of biological active compounds [2-5] . Anti-cancer activity of Betulinic acid has previously reported [6] the arilbuthanoids, diarilheptanoids, lignans and phenolic compounds, possessing high antioxidant, cytotoxic, anticancer and antiviral activities, are isolated from the different species of Betula L. [7-11] . 2. Materials and methods 2.1. Plant material The Bark of Betula megrelica L. was collected at north side of Mt. Migaria (Georgia). A herbarium specimen (No 7817) is deposed in the herbarium of the department of pharmacobotany, at the TSMU Iovel Kutateladze Institute of Pharmacochemistry (Herbarium TBPH, Tbilisi, Georgia). 2.2. Experimental procedures 2.2.1. Extraction and Isolation The barks were extracted with ethanol 95% at room temperature, then at 70 0 C with ethanol 70% (2 times). The pulled ethanol extracts were evaporated to dryness under reduced pressure. The obtained residue was repeatedly chromatographed on Diaion® HP-20 and rich fractions (H2O, MeOH 30% contained compounds 1,2, and 3; MeOH 50% contained compounds 5,6,7,8 and 9; 80% MeOH contained compound 10 and MeOH 100% contained compound 11) were obtained. Finally individual compounds from the fractions were isolated by preparative HPLC. The structures of isolated compounds were determined by 1 H, 13 C NMR (COSY, HSQC, HMBC) (Bruker Avance 400MHz). TLC analyses of isolated biological active compounds were carried out on silica gel plates (Silica gel 60 F254, Merck) using the following solvent systems:1) CHCl3-MeOH-H2O 26:14:3; 2) BuOH-HOAc-H2O 4:1:5; 3) CH2Cl2-MeOH-H2O 50:25:5. The spots were observed under UV light (254 and 365 nm) and on daylight after treatment with the reagents, the chromatograms were heated to 100-150°C. The purity of tested compounds were evaluated by TLC and analytical HPLC. 2.3. Chemicals and reagents. HPLC grade acetonitrile of analytical grade were purchased from Merck & Co. Ultrapure water (for HPLC analysis was obtained from a Millipore Classic purification system. 2.4. Chromatographic instrument and conditions. HPLC-DAD-MS analyses were performed on an Agilent 1100 series HPLC-DAD-MS system. The UV spectra were recorded from 190 to 400 nm. The chromatographic separation was