Turk J Pharm Sci 10(1), 19-24, 2013 Original article ASSESSMENT OF LEVOFLOXACIN EFFECT ON HUMAN NEUTROPHIL FUNCTIONS IN VITRO H ii mey r a CO M ERTER 1 , Tan ju KADIR 2 , Be tii l OK UY AN 1 , M e sut SANCAR 1 , Fi kr e t Ve hb i I ZZ ETTIN 1 * ' Marmara University, Faculty of Pharmacy, Clinical Pharmacy Department, 34718 Haydarpasa-Istanbul, TURKEY 2 Marmara University, School of Dentistry, Department of Microbiology, 34365 Nisantasi-Istanbul, TURKEY Abstr act The aim of the study was to determine the effects of levofloxacin on the phagocytic and intracellular killing functions of human neutrophils at different therapeutic concentrations and to investigate the possible differences between pure powder and pharmaceutical form of levofloxacin on the neutrophil functions. Neutrophils obtained from healthy volunteers were incubated with both pure powder and pharmaceutical form of levofloxacin at different concentrations (1.4 pLg/mL, 2.8 pLg/mL, 5.7 pLg/mL, 11.4 Hg/mL). At concentrations of 2.8 pLg/mL solutions, prepared from both pure powder and pharmaceutical form of levofloxacin, were observed to decrease phagocytosis and intracellular killing comparing to control samples, but only the decrease for the pharmaceutical form's effect on phagocytic function was statistically significant (p<0.05). At concentration of 2.8 pig/mL solution, prepared from pharmaceutical form of levofloxacin, was observed to statistically significant decrease phagocytosis comparing to 2.8 fig/mL solution prepared from pure powder of levofloxacin (p<0.05). At concentration of 5.7 pig/mL solution, prepared from pharmaceutical form of levofloxacin, was observed to statistically significant decrease phagocytosis and intracellular killing comparing to 5.7 pig/mL solution prepared from pure powder of levofloxacin (p<0.05). Phagocytic activity of neutrophils was significantly decreased by levofloxacin solution prepared from pure powder at concentrations of 11.4 pig/mL when compared with control (p<0.05). As conclusion, the solutions prepared with pharmaceutical form have significantly inhibition on the phagocytic and intracellular killing functions of human neutrophils in vitro when comparing with the solutions prepared with pure powder. Key words: Phagocytosis, Intracellular killing, Levofloxacin, Neutrophil. Levofloksasin’in insan Notrofil Fonksiyonlari Uzerine Etkisinin In vi tro Degerlendirilmesi Qalismamizin amaci, farkli terapotik konsantrasyonlardaki levofloksasinin; insan nötrofillernin fagositoz ve hücre igi oldurme fonksiyonlari üzerine etkilerini belirlemek ve notrofil fonksiyonlari üzerine levofloksasin'in tablet ve hammadde formlan arasindaki olasi farklan arastirmaktir. Saghkli gonullulerden elde edilen notro filler, farkli konsantrasyonlardaki (1.4 fig/mL, 2.8 fig/mL, 5.7 fig/mL, 11.4 fig/mL) hem hammadde hem de tablet formundaki levofloksasin gozeltileri He inkiibe edilmistir. 2.8 fig/mL 'lik hammadde ve tablet dozaj formunda hazirlanan gozeltiler He notrofillerin fagositoz ve Mere igi oldurme fonksiyonlannda kontrole gore azalma izlenmistir; ancak sadece 2.8 [ig/mL'lik tablet gozeltisi He notrofillerin fagositoz fonksiyonundaki azalma kontrole gore istatistiksel olarak anlamli bulunmustur (p<0.5). Levofloksasin'in 2.8 [ig/mL'lik tablet formundan hazirlanan gozeltisinin, 2.8 fig/mL hammaddeden hazirlanan gozeltisiyle karsilastinldiginda fagositozu istatistiksel olarak anlamli azalttigi bulunmustur (p<0.05). 5.7 [ig/mL'lik tablet gozeltisinin 5.7 [ig/mL'lik hammadde gozeltisine oranla notrofillerin fagositik ve Mere igi oldurme etkilerini istatistiksel olarak anlamli derecede azalttigi bulunmustur (p<0.05). 11.4 fig/mL 'lik hammadde gozeltisi He notrofillerin fagositoz fonksiyonu kontrole gore istatistiksel olarak anlamli duzeyde azalmistir (p<0.05). Sonug olarak, tablet dozaj formundan hazirlanan gozeltiler He inkiibe edilen notrofillerde, hammadde çözeltileri ile inkübe edilen nötrofillere oranla fagositoz ve Mere igi oldurme fonksiyonlari uzerine anlamli inhibisyon saptanmistir. Anahtar kelimeler: Fagositoz, Hücre içi öldürme, Levofloksasin, Nötrofil. Correspondence: E-mail: fVizzettin@hotmail.com; Tel: +90-216 346 4060 19