PRECLINICAL RESEARCH Matrix Signaling Subsequent to a Myocardial Infarction A Proteomic Profile of Tissue Factor Microparticles Derrick Akpalu, a Gale Newman, PHD, a Mark Brice, PHD, a Mike Powell, PHD, a Rajesh Singh, PHD, a Alexander Quarshie, MD, b Elizabeth Ofili, MD, b James Fonger, MD, c Nic Chronos, MD, d David Feldman, MD, PHD e,f VISUAL ABSTRACT Akpalu, D. et al. J Am Coll Cardiol Basic Trans Science. 2017;2(5):529–42. HIGHLIGHTS  The occurrence of an MI activates production of TFMPs.  We induced an MI in Yucatan miniswine and collected plasma samples over a 6-month period post-MI.  Experimental groups consisted of infarcted but untreated animals and infarcted animals treated with CRT plus b-blocker.  Using proteomic profiling, we confirm the heterogeneity of TFMP protein content with respect to physiological status of the host temporally.  Spatially, the contents of the TFMPs provided information about multiple entities supplemental to what we obtained from assessing a set of 8 currently used cardiac biomarkers.  The results from this study support recommending TFMP protein content profiling be used prospectively as a viable investigative methodology for chronic ischemic cardiomyopathy to help improve our understanding of b-adrenergic receptor signaling after an MI. From the a Department of Microbiology, Biochemistry and Immunology, Morehouse School of Medicine, Atlanta, Georgia; b Clinical Research Center, Morehouse School of Medicine, Atlanta, Georgia; c CardioScout LLC, Atlanta, Georgia; d Cardiology Care Clinic of Lake Oconee, Eatonton, Georgia; e Division of Cardiology University of Cincinnati Medical Center, Cincinnati, Ohio; and the f Department of Cardiology, Inselspital Hospital, University of Bern, Switzerland. This work was supported by the following Na- tional Institutes of Health (NIH) and National Institute on Minority Health and Health Disparities (NIMHD) grants: NIH 5RO1HL84498-5, NIH/NIMHD 2S21MD000101NIMHD 8U54MD007588-04; the Minority Biomedical Research Support of the Research Initiative for Scientific Advancement program 5R25GM058268; the Research Centers in Minority Institutions 5G12MD007602; and NIH/NHLBI contract grant number R21HL092358. All authors have reported that they have no relationships relevant to the contents of this paper to disclose. JACC: BASIC TO TRANSLATIONAL SCIENCE VOL. 2, NO. 5, 2017 ª 2017 PUBLISHED BY ELSEVIER ON BEHALF OF AMERICAN COLLEGE OF CARDIOLOGY FOUNDATION. THIS IS AN OPEN ACCESS ARTICLE UNDER THE CC BY-NC-ND LICENSE ( http://creativecommons.org/licenses/by-nc-nd/4.0/ ). ISSN 2452-302X http://dx.doi.org/10.1016/j.jacbts.2017.04.004