Atherosclerosis 181 (2005) 403–405 Postprandial increase of plasma apoAV concentrations in Type 2 diabetic patients Val´ erie Pruneta-Deloche a, ∗ , Gabriel Ponsin a , Laure Groisne b , Jamila Fruchart-Najib c , Michel Lagarde a , Philippe Moulin a, b a UMR 585 INSERM/INSA, Physiopathologie des Lipides et Membranes, Villeurbanne, France b Unit´ e11,Hˆ opital Cardiovasculaire Louis Pradel, Lyon-Bron, France c U 545 INSERM, Institut Pasteur Lille, Lille, France Received 26 October 2004; received in revised form 7 January 2005; accepted 25 January 2005 Available online 17 February 2005 Abstract Postprandial hypertriglyceridemia is considered as a risk factor for cardiovascular disease in Type 2 diabetes. However, little is known about the underlying mechanisms. Since the recently discovered apolipoprotein (apo) AV was identified as a modulator of triglyceride (TG) metabolism, the aim of the study was to determine the postprandial apoAV profile of Type 2 diabetic patients. We compared data from 11 patients with Type 2 diabetes mellitus to that of 12 non-diabetic normolipidemic subjects following the ingestion of a lipid-rich cream. Postprandial apoAV was elevated in diabetic patients but no correlation was observed either with plasma TG concentration or with the intensity of lipoprotein lipase-dependent lipolysis. These data obtained in human subjects suggest that plasma apoAV concentration does not play an acute or a direct role in the regulation of plasma TG in the postprandial state. © 2005 Elsevier Ireland Ltd. All rights reserved. Keywords: Postprandial lipemia; Type 2 diabetes; Apolipoprotein AV; Lipolysis 1. Introduction Hypertriglyceridemia in the fasting and the postprandial states is a prominent feature of dyslipidemia in both Type 2 diabetes and metabolic syndrome, and it is considered to be an independent risk factor for coronary heart disease [1]. The mechanisms of this secondary hypertriglyceridemia involve a reduction of lipoprotein lipase (LPL) activity, an overproduc- tion of triglyceride (TG)-rich lipoproteins and a defect in the plasma clearance of remnants. The contribution of new mod- ulators of plasma TG concentrations such as apolipoprotein AV (apoAV) needs to be investigated. Several human stud- ies evidenced an association between several polymorphisms within the apoAV locus and plasma TG levels or VLDL TG ∗ Corresponding author. Tel.: +33 4 72 43 85 24; fax: +33 4 72 43 85 24. E-mail address: valerie.pruneta-deloche@insa-lyon.fr (V. Pruneta-Deloche). concentrations [2–6]. Transgenic mouse models and aden- oviral overexpression established that apoAV was decreasing plasma TG concentration [2,7,8]. It has been proposed that apoAV was capable to decrease both the lipidation of TG- rich lipoproteins in hepatocytes and to stimulate LPL activity, two major determinants of postprandial hypertriglyceridemia [7,9]. These findings led us to investigate the putative rela- tionship between plasma apoAV concentrations and whether the postprandial hypertriglyceridemia observed in Type 2 di- abetes could be at least partially explained on the basis of a putative decrease of plasma apoAV concentrations. 2. Subjects and methods Eleven male patients with Type 2 diabetes mellitus were compared with 12 non-diabetic, normolipidemic healthy male controls matched for age. All subjects gave written in- formed consent to the study protocol which was approved 0021-9150/$ – see front matter © 2005 Elsevier Ireland Ltd. All rights reserved. doi:10.1016/j.atherosclerosis.2005.01.034