Supplementary data In Vivo Visualization of Osteoarthritic Hypertrophic Lesions Hai-Yu Hu 1,2,5 , Ngee-Han Lim 3 , Hans-Paul Juretschke 2 , Danping Ding-Pfennigdorff 2 , Peter Florian 2 , Markus Kohlmann 2 , Abdulah Kandira 2 , Jens Peter von Kries 4 , Joachim Saas 2 , Karl A. Rudolphi 2 , K. Ulrich Wendt 2 , Hideaki Nagase 3 , Oliver Plettenburg 2 , Marc Nazare 2,4* , Carsten Schultz 1 * 1 European Molecular Biology Laboratory (EMBL), Interdisciplinary Chemistry Group, Cell Biology and Biophysics Unit, Meyerhofstr. 1, 69117 Heidelberg, Germany 2 Sanofi-Aventis Deutschland GmbH, Industriepark Höchst, 65962 Frankfurt, Germany 3 Kennedy Institute of Rheumatology, University of Oxford, Roosevelt Drive, Headington, Oxford OX3 7FY, United Kingdom 4 Leibniz-Institut für Molekulare Pharmakologie (FMP), Campus Berlin-Buch, Robert- Roessle-Str. 10, 13125 Berlin, Germany 5 State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, 1 Xiannongtan Street, 100050 Beijing, China. *Correspondence should be addressed to C.S. (schultz@embl.de) or M.N. (nazare@fmp- berlin.de) Contents 1. Abbreviations and general methods ---------------------------------------------------------S2 2. Synthesis of contrast agents ------------------------------------------------------------------S3 3. Histopathological score of joint damage in the rat ACLT- pMx OA model ----------S11 4. Spin-lattice relaxivity (r 1 ) measurement of MRI contrast agents --------------------S15 5. Ex vivo MRI Studies with pig cartilage explants ----------------------------------------S16 6. In vivo MRI studies with rats --------------------------------------------------------------S17 7. Immunofluorescence staining for type IIA procollagen --------------------------------S21 8 Histological studies of non-targeting control probe CP------------------------------S22 9. References---------------------------------------------------------------------------------------S23 Electronic Supplementary Material (ESI) for Chemical Science. This journal is © The Royal Society of Chemistry 2015