_____________________________________________________________________________________________________ *Corresponding author: E-mail: lawal.almustapha@udusok.edu.ng; International Journal of Biochemistry Research & Review 31(1): 1-8, 2022; Article no.IJBCRR.84481 ISSN: 2231-086X, NLM ID: 101654445 Effects of Sub-chronic Administration of Crude Ethanol Extract of Cola nitida on Stomach Mucosa Epithelial Lining and Liver Function Enzymes in Albino Rats Almustapha Lawal a* , Mu’azu Gusau Abubakar a , Aminu Lailaba Abubakar a , Sadiq Mas’ud Eneji a , Rabiu Sulaiman a , Ebuka Okafor a and Wali Usman b a Department of Biochemistry and Molecular Biology, Faculty of Science, Usmanu Danfodiyo University, Sokoto, P.M.B-2346, Sokoto State, Nigeria. b Department of Chemical Pathology, School of Medical Laboratory Science, Usmanu Danfodiyo University, Sokoto, P.M.B-2346 Sokoto, Nigeria. Authors’ contributions This work was carried out in collaboration among all authors. All authors read and approved the final manuscript. Article Information DOI: 10.9734/IJBCRR/2022/v31i130296 Open Peer Review History: This journal follows the Advanced Open Peer Review policy. Identity of the Reviewers, Editor(s) and additional Reviewers, peer review comments, different versions of the manuscript, comments of the editors, etc are available here: https://www.sdiarticle5.com/review-history/84481 Received 01 November 2021 Accepted 03 January 2022 Published 10 January 2022 ABSTRACT Aim: This study investigated the effects of sub-chronic administration of crude ethanol extract of Cola nitida mucosa epithelial lining and liver function enzymes in albino rats. Place and Duration of Study: Department of Biochemistry and Molecular Biology and Chemical Pathology Usmanu Danfodiyo University, Sokoto, between March 2017 and January 2018. Methodology: Twenty (20) albino rats have randomly divided into five (5) groups of A, B, C, D, and E of four rats each and were fed with an equal volume of ethanol extract of Cola nitida of 600, 1200, 1800 and 2400 mg/kg body weight of the rats by oral administration for 28 days, while group A serves as a control, respectively. Results: Result indicates the LD50 was above 5000 mg/kg B.W., serum aspartate transaminase (AST) and alanine transaminase (ALT) activity revealed a significant increase (P = .05) for AST (31.23 ± 9.39), (40.44 ± 12.24), (44.59 ± 8.69), and (36.30 ± 13.18) in rats fed with 600, 1200, 1800 and 2400 mg/kg, and ALT (33.66 ± 7.94) for group fed with 2400 mg/kg B.W as compared against Original Research Article