Environ Biol Fish DOI 10.1007/s10641-015-0428-y Spatial and temporal movement dynamics of brook Salvelinus fontinalis and brown trout Salmo trutta Lori A. Davis · Tyler Wagner · Meredith L. Bartron Received: 10 October 2014 / Accepted: 12 June 2015 © Springer Science+Business Media Dordrecht 2015 Abstract Native eastern brook trout Salvelinus fonti- nalis and naturalized brown trout Salmo trutta occur sympatrically in many streams across the brook trout’s native range in the eastern United States. Understand- ing within- among-species variability in movement, Electronic supplementary material The online version of this article (doi:10.1007/s10641-015-0428-y) contains supplementary material, which is available to authorized users. Disclaimer: This draft manuscript is distributed solely for purposes of scientific peer review. Its content is deliber- ative and predecisional, so it must not be disclosed or released by reviewers. Because the manuscript has not yet been approved for publication by the US Geological Survey (USGS), it does not represent any official finding or policy. L. A. Davis () Pennsylvania State University, University Park, PA 16802, USA e-mail: Lori Davis@fws.gov; loriadavis78@gmail.com T. Wagner U.S. Geological Survey, Pennsylvania State University, University Park, PA 16802, USA M. L. Bartron U.S. Fish and Wildlife Service, U.S. Fish and Wildlife Service, Northeast Fishery Center, Lamar, PA 16848, USA Present Address: L. A. Davis U.S. Fish and Wildlife Service, Northeast Fishery Center, Lamar PA, USA including correlates of movement, has implications for management and conservation. We radio tracked 55 brook trout and 45 brown trout in five streams in a north-central Pennsylvania, USA watershed to quantify the movement of brook trout and brown trout during the fall and early winter to (1) evalu- ate the late-summer, early winter movement patterns of brook trout and brown trout, (2) determine corre- lates of movement and if movement patterns varied between brook trout and brown trout, and (3) evalu- ate genetic diversity of brook trout within and among study streams, and relate findings to telemetry-based observations of movement. Average total movement was greater for brown trout (mean ± SD = 2,924 ± 4,187 m) than for brook trout (mean ± SD = 1,769 ± 2,194 m). Although there was a large amount of among-fish variability in the movement of both species, the majority of movement coincided with the onset of the spawning season, and a threshold effect was detected between stream flow and movement: where movement increased abruptly for both species during positive flow events. Microsatellite analysis of brook trout revealed consistent findings to those found using radio-tracking, indicating a moderate to high degree of gene flow among brook trout popula- tions. Seasonal movement patterns and the potential for relatively large movements of brook and brown trout highlight the importance of considering stream connectivity when restoring and protecting fish popu- lations and their habitats.