INTERNATIONAL JOURNAL OF SCIENTIFIC & TECHNOLOGY RESEARCH VO`LUME 10, ISSUE 03, MARCH 2021 ISSN 2277-8616 212 IJSTR©2021 www.ijstr.org Effect Of Gembili Tuber Inuline (Dioscorea Esculeta) On Trygliceride Levels In White Rat Blood Serum (Rattus Norvegicus) Ari Yuniastuti, Yasinta Ayu Widyawati, Aditya Marianti, Wulan Christijanti Abstract:- Gembili tubers are a type of inferior tuber whose consumption has decreased or even decreased. People do not know that Gembili tubers (Dioscorea esculenta) contain useful inulin. Reducing triacylglycerol and preventing degenerative diseases such as coronary heart disease and diabetes mellitus. This study was to determine the effect of giving inulin content to gembili tubers on serum of hypercholestrolemic wistar rats found in gembili tubers. This research is included in a laboratory study conducted on white rats aged 2- 3 months, body weight 150-180 grams. Rats were divided into 6 groups, namely the positive control group, the negative control group, the group treated with simvastatin induction, the treatment group with doses of 100,200 and 400 mg / kgBW and the treatment of high cholesterol fat feed induction for 14 days along with the administration of inulin extract. On the 15th day, the rat's blood sample was taken via retroorbitalis, then the Triglyceride level was tested using the GPO-PAP method. The data were then analyzed statistically using the SPSS statistics application with a standard significance value of p <0.05. The results showed a significant difference between the inulin extract dosage of 100mg / Kg / BW, inulin extract of 200mg / kg and showed no significant calculation results between mice treated with the inulin extract dose of 400mg / KgBW and the group of mice induced with simvastatin . This can be concluded that the inulin content in the extract of the gembili tuber which has a dose level of 400mg / kgBW can reduce Triacylglycerol levels in the body. inulin extract of 200mg / kg and showed no significant calculation results between mice treated with the inulin extract dose of 400mg / kgBW and the group of mice induced with simvastatin. This can be concluded that the inulin content in the extract of the gembili tuber which has a dose level of 400mg / kgBW can reduce Triacylglycerol levels in the body. Inulin extract of 200mg / kg and showed no significant calculation results between mice treated with the inulin extract dose of 400mg / kgBW and the group of mice induced with simvastatin. This can be concluded that the inulin content in the extract of the gembili tuber at dose level of 400mg / kgBW can reduce triacylglycerol levels in the body. Index Terms:- blood serum, gembili tubers (Dioscorea esculenta), hypercholestrolemic ,inulin, triglycerides, Rattus Norvegicus. ——————————  —————————— 1 INTRODUCTION Inulin is a functional bioactive compound that is often used in the medical and pharmaceutical world because it has various benefits, such as being able to normalize blood sugar levels in diabetics and reduce the risk of colon cancer [1]. The need for inulin in Indonesia is still imported, considering that inulin is produced by the cicory plant, where this plant is not found in Indonesia. Several studies have been conducted to produce inulin from local natural ingredients in Indonesian regions. One of them is from the gembili tuber. The inulin content of gembili is 1.53 g / mg of gembili flour [1], while the results of isolation and identification of inulin from gembili tubers produce a solid composition of 3.983%, reducing sugar is 3.3458 mg / mL, 19.9098% inulin, and 2.0667 mg / mL of total sugar [2]. Gembili tubers are a type of inferior tuber whose consumption has decreased or even decreased, due to a lack of information about the benefits of gembili tubers for health, so that the cultivation of gembili has also decreased. Gembili is generally used as one of the staple food sources because it has a high carbohydrate content. That the largest component of the gembili tuber is carbohydrate 27-33%. In 100 g of gembili tubers there are 31.30 grams of carbohydrates [3]. However, besides being high in carbohydrates, gembili tubers also have a high enough inulin content. The benefits of inulin include anti-diabetics, anti-hyperlipidemia (decreases fat levels), and anticancer. The benefits of inulin gembili as antihyperlipidemia have not been widely reported. High levels of triacylglycerol (triglycerides) lead to the risk of other vascular disease. Therefore, it is hoped that inulin gembili can reduce levels of triacylglycerols or triglycerides, so that degenerative diseases and vascular diseases can be managed properly and their further development can be prevented. Increased levels of triglycerides in the blood can increase the risk of coronary heart disease and metabolic syndrome. An increase in triglyceride levels by 1 mmol / L can increase the risk of cardiovascular disease in men by 30% and in women by 75% [4]. Based on the 2013 Basic Health Research, it shows the proportion of population ≤ 15 years old with triglyceride levels above the normal value, namely 13.0% [4]. Inulin as a prebiotic can reduce triglyceride levels by inhibiting the activity of lipogenic enzymes in synthesizing triglycerides in the liver. Lipogenic enzymes consist of acetyl coenzyme A (coA), Malic enzyme, ATP citrate lyase, and Fatty acid synthase. Inulin can inhibit the expression of the mRNA gene in regulating the activity of the fatty acid synthase enzyme, so that the activity of the fatty acid synthase enzyme is inhibited, causing the regulation of triglyceride formation in the liver to be inhibited [5]. Therefore, a study aimed to determine the effect of inulin extract from gembili tubers (Dioscorea esculeta) on triglyceride levels in blood serum of white rats (Rattus norvegicus), using the measurement method using the GPO- PAP Kit (Glyserol Peroxidase Phosphate Acid) and a spectrophotometer at 500nm wavelength. 2 MATERIALS AND METHODS The research subjects of this study were the white rat strain Wistar (Rattus norvegicus), then they were kept and fed normal food. The wistar rats used were healthy rats and had normal activity, male sex, 2 to 3 months old and weighing about 150-180 grams. The total number of mice used was 24 rats. Rats were divided into 6 treatment groups, namely Normal group, Positive Control (simvastatin drug) negative control (without inulin), treatment 1 (dose 100 mg), treatment 2 ————————————————  Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang, Semarang, Central Java, Indonesia  Corresponding author: A. Yuniastuti, ariyuniastuti@mail.unnes.ac.id  ORCID iD : 0000-0002-3574-9180