_____________________________________________________________________________________________________ *Corresponding author: E-mail: hishamwagg30@hotmail.com; Journal of Pharmaceutical Research International 33(60B): 192-200, 2021; Article no.JPRI.78649 ISSN: 2456-9119 (Past name: British Journal of Pharmaceutical Research, Past ISSN: 2231-2919, NLM ID: 101631759) The Potential Effect of Henna (Stone Dye) Extract Paraphenylenediamine on Human Blood Cells, Liver and Renal Function Umsalma B. Eissa a , Fathia Khogali b , Elamin E. Mohamed c , Elbadri E. Osman d , Osman Mohammed Elmahi e and Hisham Ali Waggiallah f* a Faculty of Productional Technology and Agricultural Processing, International University of Africa, Khartoum, Sudan. b Faculty of Animal Production, Sudan University for Science and Technology, Khartoum, Sudan. c Faculty of Science, University of Khartoum, Khartoum, Sudan. d Faculty of Pure and Applied Science, International University of Africa, Khartoum, Sudan. e Department Histopathology and Cytology, Faculty of Medical Laboratory Sciences, Karary University, Khartoum, Sudan. f Department of Medical Laboratory Science, College of Applied Medical Science, Prince Sattam Pin Abdulaziz University, KSA. Authors’ contributions This work was carried out in collaboration among all authors. All authors read and approved the final manuscript. Article Information DOI: 10.9734/JPRI/2021/v33i60B34604 Open Peer Review History: This journal follows the Advanced Open Peer Review policy. Identity of the Reviewers, Editor(s) and additional Reviewers, peer review comments, different versions of the manuscript, comments of the editors, etc are available here: https://www.sdiarticle5.com/review-history/78649 Received 09 October 2021 Accepted 19 December 2021 Published 21 December 2021 ABSTRACT Some of the dyes that are used for coloring hair and skin contain a chemical compound called paraphenylenediamine (PPD), which is potentially toxic. The study comprised 50 adult Sudanese women who were selected by stratified random sampling and working as henna artist. Urine samples were taken for detection of PPD while blood samples were taken for investigating kidney and liver functions by measuring its relevant hematological indices. In other hand Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC) were used to detection of PPD in black henna (stone dye) samples and urine. The PPD was detected in the urine of all women who used the black henna its concentration varied according to duration of exposure being significantly higher in those with >10 years of exposure (p≤0.01). Similarly significant Original Research Article