Abstract Food-borne pathogens is a group of micro-organisms that cause food-borne illness, thus the research for finding effective drugs against this infection is necessary. The purpose of this study determines antibacterial, antioxidant activity antifungal of bee glue ethanol and aqueous extracts. Antibacterial and antifungal activities of extracts were evaluated against food-born (Escherichia coli, Staphylococcus aureus, Bacillus cereus, Vibrio cholerae and Candida albicans) purchased from Boali hospital on by the well diffusion method. Broth serial dilution method was doing to find the Minimal Inhibitory Concentration (MIC) and the agar dilution method to find the Minimal Bacterial Concentration (MBC) of extracts. The antioxidant activity of the extracts assay- ing by 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Ethanol extract of bee glue was found the most effective against S. aureus strains (inhibition zone=27±0.87 mm) than E. coli strains (inhibi- tion zone=5.5±0.37mm). Aqueous extract of propolis was found the most effective against S. aureus strains (inhibition zone=14.5±0.75 mm) than E. coli strains (inhibition zone=3.5±0.5mm) and C. albicans (inhibition zone=10±0.5 mm). The MIC and MBC values of the extracts ethanol and aqueous most effective against B. cereus strains were 0.3125, 0.625, 0.3125 and 1.25 mg/mL than E. coli strains 0.625, 1. 25, 2.5 and 50 mg/mL. and also for C. albicans was 0.625, 1.25, 1.25 and 2.5 mg/mL. IC50 ethanol and aqueous extracts of propolis were 8±0.25 and 45±1.75, respectively. This study was to assess the Antibacterial, antifungal and antioxidant activities activities of bee glue extracts in in vitro. Introduction The honey bees can be considered as the collect bee glue from plant resin through biting off scraps. Bee glue is hard and fragile at room temperature and soft, malleable and sticky when heated. It can be extracted by the honey bees from the resin of plants in the inside of bee nest and used to repair honeycombs, and keep up disinfection of the beehive, prevent decay organ- isms inside the bee nest that dies. 1,2 Furthermore, the variation in the composition of bee glue can be associated with the plant species, regional geography, and seasons, which were visited by the honey bees as well as the metabolized and secreted sub- stances by them and also further added components during its production. 3 Some biological properties such as antioxidant, antifungal, and antimicrobial activity, mainly due to of phenolic com- pounds, especially flavonoids, were demonstrated previously. 4-6 Moreover, recently, the utilization of natural preservative ingre- dients such as natural waxes, plant extracts and essential oils in order to inhibition of growth of foodborne pathogens as well as spoiling molds in food products is highly regarded. 7-8 Due to favorable properties, bee glue can be utilized in variety of appli- cations, such as anti-wrinkle, anti-acne, anti-inflammatory, anti- viral, anti-diabetic and leishmanicidal agents. 9-17 In addition approaching of extracted bioactive compounds from medicinal plants sources as antimicrobial and antioxidants agents attracted notable attention. 18,19 In this context, the bee glue extracts can be incorporated in food processing, nutraceuticals or cosmetic anti- aging formulations. Hence, the goal of this study was undertaken to assess the antioxidant and/or antimicrobial activities of bee glue extracts an in vitro system. Correspondence: Amir Sasan Mozaffari Nejad, Hamadan University of Medical Sciences, Hamadan, Iran. E-mail: as.mozafarinejad@umsha.ac.ir Key words: Bee glue, antioxidant activity, antimicrobial activity, extract. Contributions: the authors contributed equally. Conflict of interest: the authors declare no potential conflict of interest. Acknowledgements: the authors would like to acknowledge the finan- cial support of the Food Safety and Chemical Research Center, Tehran University of Medical Science, Iran. Received for publication: 7 December 2016. Revision received: 25 April 2017. Accepted for publication: 5 May 2017. ©Copyright N. Shariatifar et al., 2017 Licensee PAGEPress, Italy Journal of Biological Research 2017; 90:6452 doi:10.4081/jbr.2017.6452 This article is distributed under the terms of the Creative Commons Attribution Noncommercial License (by-nc 4.0) which permits any non- commercial use, distribution, and reproduction in any medium, provid- ed the original author(s) and source are credited. [Journal of Biological Research 2017; 90:6452] [page 77] Journal of Biological Research 2017; volume 90:6452 Antibacterial, antifungal and antioxidant activities of bee glue ethanol and aqueous extracts Nabi Shariatifar, 1 Akram Janghorban, 2 Rezvan Rahimnia, 3 Amir Sasan Mozaffari Nejad 4 1 Department of Environmental Health, School of Public Health, Tehran University of Medical Sciences, Tehran; 2 Department of Food Industry, Islamic Azad University, Science and Research Branch, Tehran; 3 Department of Environmental Education, School of Humanities, Payame Noor University, Tehran; 4 Nutrition Health Research Center, Student Research Center, Hamadan University of Medical Sciences, Hamadan, Iran. Non-commercial use only