TEJAS Thiagarajar College Journal ISSN (Online): 2456-4044 January 2016, Vol 1(1), PP 88 - 98 88 Fabrication Of Wound Dressings Impregnated With Zinc Oxide Nanoparticles Synthesized From Honey: A Facile Approach For Antibacterial Application C.Usha, S.Lok Kirubahar, M. Karthikeyan and Rm. Murugappan Department of Zoology & Microbiology, Thiagarajar College, Madurai Abstract The present study explores the fabrication of wound dressing materials impregnated with zinc oxide nanoparticles (ZnO NPs) using honey as a reducing and capping agent. Formation of Honey-zinc oxide nanoparticles (H-ZnO NPs) was confirmed by surface plasmon resonance absorption at 360 nm. Morphology and elemental composition of the synthesized H-ZnO NPs were analysed by scanning electron microscopy and energy dispersive X-ray spectroscopy. The wound dressing material was impregnated with H-ZnO NPs by pad-dry cure method. The biocompatibility of the ZnO NPs loaded dressing material was evaluated by hemolysis (1.18%) and whole blood clotting test. The antibacterial activity of the dressing materials was assessed qualitatively and quantitavely by AATCC technique. The ZnO NPs loaded dressing materials possessed a remarkable antibacterial activity compared to honey impregnated and untreated dressing materials. The results illustrate that the biofabricated wound dressing material can be efficiently exploited in therapeutic application. Keywords: zinc oxide nanoparticles, honey, hemocompatibility, wound healing, biofabrication * Corresponding author: Tel: +91-9443918665 (mobile) E-mail address:hod_zoology@tcarts.in Introduction Recently, there has been a tremendous surge in unraveling the potential application of metal oxide nanoparticles in biomedical application. Of the different metals, utilization of zinc in nanoparticle synthesis is meager. Nanosized ZnO is an highly preferred multitasking metal oxide due to its unique optical and electrical properties (Wang et al., 2004). Furthermore, zinc oxide is