RESEARCH ARTICLE Am. J. PharmTech Res. 2014; 4(4) ISSN: 2249-3387 Please cite this article in press as: Nagavi J et al., Analytical RP-HPLC Method Development and Validation for The Simultaneous Estimation of Ramipril and Hydrochlorothiazide in Tablet Dosage Form. American Journal of PharmTech Research 2014. Analytical RP-HPLC Method Development and Validation for the Simultaneous Estimation of Ramipril and Hydrochlorothiazide in Tablet Dosage Form Jinesh Bahubali Nagavi 1* , Preethi Gotadake Anantharaju 2 . 1Department of Pharmaceutical Chemistry, JSS College of Pharmacy, JSS University, Mysore 570015, Karnataka, India. 2Department of Biochemistry, JSS Medical College, JSS University, Mysore 570015, Karnataka, India. ABSTRACT A HPLC method for simultaneous estimation of Ramipril and Hydrochlorothiazide in tablets was developed and validated. The developed method involves Purosphere ® Star Rp18e, 5μm, 150×4.6mm column with mobile phase composition of acetonitrile and sodium perchlorate (pH 2.5) buffer in the ratio of 3:2, at a flow rate of 1.0 ml/min and UV detection at 316nm for first five minutes for Hydrochlorothiazide and 210nm for Ramipril. The method was validated as per ICH guidelines, Linearity was observed over concentration range of 17.5 to 32.5 μg/ml for Ramipril and 87.5 to 162.5 μg/ml for Hydrochlorothiazide. The Accuracy of the proposed method was determined by recovery studies and found to be 97.95-102.3% and 97.98-102.66% for Ramipril and Hydrochlorothiazide respectively. The proposed method was extended for estimation of Ramipril and Hydrochlorothiazide in marketed tablet formulation (Ramace-H TM ) and it was found to be well within the acceptance limit. The developed and validated HPLC method for simultaneous estimation of Ramipril and Hydrochlorothiazide was found to be linear, accurate, precise, robust and rugged. Hence it can be used for routine analysis of Ramipril and Hydrochlorothiazide in tablets. Keywords: HPLC, Ramipril, Hydrochlorothiazide, Ramace-H TM . *Corresponding Author Email: nagavi.jinesh@gmail.com Received 22 August 2014, Accepted 28 August 2014 Journal home page: http://www.ajptr.com/