COMPARATIVE PHYTOCHEMICAL ANALYSIS OF IN VITRO AND IN VIVO PLANT PARTS OF OROXYLUM INDICUM (L.). VENT. – AN ENDENGERED MEDICINAL PLANT Illa Patel* & Esha Rami Department of Life Science, Hemchandracharya North Gujarat University Patan, (Gujarat) 384265 – India Received on 02 – 03 - 2013 Revised on 19 – 03- 2013 Accepted on 23– 04 – 2013 ABSTRACT Oroxylum indicum (L.) Vent. is used extensively in the preparation of both traditional and modern medicines. In present study, comparative account of phytochemical screening of methanolic extract was performed in in vivo plant parts like leaf, stem bark and root and in vitro developed Callus. Callus was cultured on Murashige and Skoog (MS) medium supplemented with BAP (2mg/L) and IAA (0.2mg/L). Result obtained from in vivo and in vitro phytochemical screening of methanolic extract shows the presence of flavonoids, alkaloids, saponin, tannins, glycosides, phenol, that show wide medicinal properties. Root bark shows better accumulation of secondary metabolites in any other plant parts and callus. Key Words: Oroxylum indicum (L.) Vent. ,phytochemicals screening, TLC analysis. INTRODUCTION Medicine plant has been proved to be the most useful in treatment of many diseases since from ancient time as they provide an important source of most of the drugs in the world. Medicinal herbs are moving from fringe to mainstream use with a great number of people seeking remedies and health approaches free from side effects caused by synthetic chemicals [1] . Plant produces a variety of secondary metabolites that have been a source of medicine. These metabolites are being used, as precursors or as lead compound in the pharmaceutical industry for making various drugs against many diseases. Address For Correspondence Illa patel Department of Life Science, Hemchandracharya North Gujarat University, Patan, (Gujarat) - 384 265, India illa.botany@gmail.com Oroxylum indicum (L.) Vent. Family Bignoniaceae is commonly called as Sonapatha, Syonaka and Midnight horror which is widely found in India, shri lanka, china, Philippines and Malaysia [2] . This plant is a very important herbal medicine in many Asian countries and is used in traditional medicine as a cure of various diseases [3] . This plant possesses a flavonoid viz. Baicalein used to check proliferation of human breast cancer cell line MDA - MB - 435 [4] . This plant possesses antioxidant, antifungal, antimicrobial, anti-inflammatory, antibacterial, anti- arthritic, anti-cancer property [5] . Root extract of this tree has been used for long in Ayurvedic preparations like Dashmularisht and Chyawanprash [6] . It is also one of the important ingredients in ayurvedic formulation such as Amartarista, Dantyadyarista, Narayana taila, Dhanawantara ghrita, Brahma rasayana, Awalwha, Chyavanprasha [7] . Because of its indiscriminate collection and over exploitation for medicinal purpose has pushed this plant to the list of endangered plant species of India [8] . The present investigation is aimed at screening both in vivo and In-Vitro developed samples for their biological compound. Research Paper ISSN: 2230 – 7583 Illa Patel, et al. / International Journal of Advances in Pharmaceutical Research IJAPR Available Online through www.ijapronline.org IJAPR /May. 2013/ Vol. 4 /Issue. 5 / 1701 – 1705 1701