Analele ştiinţifice ale Universităţii “Al. I. Cuza” Iaşi Tomul LV, fasc. 1, s.II a. Biologie vegetală, 2009 CHEMICAL COMPOUNDS IDENTIFIED IN THE LEAF GLANDS OF ROSA AGRESTIS SAVI AND ROSA RUBIGINOSA L. LIDIA ADUMITRESEI * , IRINA GOSTIN ** , CLARA APROTOSOAIE *** , A. ŞPAC *** , IRINA STĂNESCU * , C. TOMA ** Abstract. The multicellular clavate glands observed in the adaxial face of the leaf, the edge of the leaf, the rachis and stipules elaborate volatile oils whose chemic composition changes at the same time with springs’ maturation. The main components are: monoterpenes, aliphatic and aromatic compounds. Key words: R. agrestis, R. rubiginosa, chemic compounds, micromorphology Introduction Rosa agrestis Savi and Rosa rubiginosa L. belong to Caninae section, Rubiginosae subsection and are called roses with apple odour, near by other species, as R. elliptica and R. micrantha [1, 11]. The apple odour comes from the acetic aldehyde which is present in the glands of the leaf, but the chemic composition of those glands is more complex, as it is in other species which present glands on the leaf [4, 5, 10]. The present paper underlines micromorphologic aspects of the glands belonging to the mentioned species, on one hand, and their chemic composition, on the other hand. Material and methods The material subjected to the analysis collected from “Anastasie Fătu” Botanical Garden of Iasi belongs to Rosa agrestis Savi and Rosa rubiginosa L. Isolation of essential oil: The volatile oils were obtained from fresh vegetal material (leaves) by hydrodistillation in Clevenger apparatus (3 hours). The oil was separated, dried over anhydrous sodium sulfate and kept in a dark glass bottle at 4°C for the analysis. Oil analysis: The constituents of the essential oils have been characterized using gas chromatograph and mass spectroscopy analysis (GC-MS). GC-MS analysis: An analytical system, consisting of HP 5890 Series II GC and HP 5971 MSD, was used. GC/MS analysis of the essential oil was performed on HP-5MS capillary column (30 m x 0.25 mm diameter x 0.25 µm thick) coated with cross-linked methyl silicone gum. Carrier gas was helium with 1ml/min flow rate. Temperature program: 35°C held for 5 min, then heated up to 260°C at 10°C per min, and maintained for 3 additional minutes at this temperature. The temperature of injector was 250°C and of the MS interface was 280°C. Injection volume was 0,3 μl essential oil in hexane (S1-S3 * ‘Anastasie Fatu’ Botanical Garden of Iasi, ‘Al. I. Cuza’ University, lidia.adumitresei@yahoo.com , irinastanescu2005@yahoo.com ** Faculty of Biology, ‘Al. I. Cuza’ University, irinagostin@yahoo.com , ctoma@uaic.ro *** Faculty of Pharmacy, ‘Gr. T. Popa’ Medicine and Pharmacy University, anaclara70@yahoo.com , adi_spac@yahoo.com 39