1 Synaptotagmins Maintain Diacylglycerol Homeostasis at Endoplasmic Reticulum- Plasma Membrane Contact Sites during Abiotic Stress Noemi Ruiz-Lopez* 1+ , Jessica Pérez-Sancho 1,2b+ , Alicia Esteban del Valle 1 , Richard P. Haslam 3 , Steffen Vanneste 4,5 , Rafael Catalá 6 , Carlos Perea-Resa 6c , Daniël Van Damme 4,5 , Selene García-Hernández 1 , Armando Albert 7 , José Vallarino 1 , Jinxing Lin 8 Friml 9 , Alberto P. Macho 2 , Julio Salinas 6 , Abel Rosado 10 , Johnathan A. Napier 3 , Vitor Amorim-Silva 1 and Miguel A. Botella* 1a 1 Departamento de Biología Molecular y Bioquímica. Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”, Universidad de Málaga-Consejo Superior de Investigaciones Cientí- ficas (IHSM-UMA-CSIC), Universidad de Málaga, Campus de Teatinos, 29071 Málaga, Spain. 2 Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences; Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 201602, China. 3 Plant Sciences, Rothamsted Research, Harpenden, AL5 2JQ, UK. 4 Ghent University, Department of Plant Biotechnology and Bioinformatics, Technologiepark 71, 9052 Ghent University, Ghent, Belgium. 5 VIB Center for Plant Systems Biology, Technologiepark 71, 9052 Ghent, Belgium. 6 Departamento de Biotecnología Microbiana y de Plantas; Centro de Investigaciones Biológicas Margarita Salas-CSIC; Ramiro de Maeztu 9, 28040 Madrid, Spain. 7 Departamento de Cristalografía y Biología Estructural, Instituto de Química Física “Rocasolano”, Consejo Superior de Investigaciones Científicas, E-28006 Madrid, Spain 8 College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. 9 Institute of Science and Technology (IST), Klosterneuburg 3400, Austria. 10 Department of Botany, The University of British Columbia, Vancouver, BC V6T 1Z4, Canada. + These authors contributed equally to this work. *Corresponding authors: Miguel A. Botella (mabotella@uma.es ) & Noemi Ruiz-Lopez (noemi.ruiz@uma.es ) a Lead contact: Miguel A. Botella. b Present address: Laboratoire de Biogenèse Membranaire, UMR5200, CNRS, Université de Bordeaux, 33140, Villenave d’Ornon, France c Present address: Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA. Department of Genetics, Harvard Medical School, Boston, MA 02115, USA was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint (which this version posted July 29, 2020. ; https://doi.org/10.1101/2020.07.28.222919 doi: bioRxiv preprint