289 Jurnal Biologi Indonesia 8(2): 289-302 (2012) Performa Perangkat Diagnostik Elisa Toksoplasmosis pada Serum Domba dan Manusia Didik T Didik T. Subekti 1 , Lisda Hayati 2 & Sujud M. Raharja 3 1 Balai Besar Penelitian Veteriner, Bogor 16114, 2 Fakultas Kedokteran, Universitas Lambung Mangkurat, Banjarmasin, 3 Dinas Kesehatan Kabupaten Lumajang. Email: subekti.vmd@lycos.com ABSTRACT Performance of ELISA Toxoplasmosis Diagnostic Kits on Sheep and Human Sera Toxoplasma seropositivity in Indonesia have a high prevalence, both in human and animals. Unfortunately, the availability of diagnostic tools to support dynamic surveillance are limited. Recently, the diagnostic tools for toxoplasmosis, namely ELISA BM were developed. The technology was based on ELISA technique using soluble tachyzoite antigen from tachyzoites of Toxoplasma gondii. Kit performance is one of the important issue for acceptance of diagnostic tools prior to wide application. The purpose of the studies was to asses the quality of diagnostic tools performances. The assesment comprises of four stages. First stage was to evaluate the performance of ELISA BM compared to Latex Agglutination Test (LAT) on sheep sera. Secondly, to evaluate the performance of ELISA BM to descriminate true seropositive and seronegative toxoplasmosis on human sera. The last stage were comparing ELISA BM, ELISA TL (commercial kit) and LAT on predetermined and unknown human sera. The results show that the accuracy of ELISA BM is slightly better than ELISA TL. Agreement of ELISA BM with LAT was better againts ELISA TL with LAT. However, all performance as determined using Cohen’s κ and Gwet’s AC 1 of ELISA BM, ELISA TL and LAT were good up to very good agreement. Keywords: Toxoplasmosis, ELISA, Latex Agglutination, inter reliability agreement. PENDAHULUAN Kasus toksoplasmosis secara serologis di beberapa wilayah Indonesia cukup tinggi, baik pada manusia (> 40 %) maupun hewan yang prevalensinya bervariasi mulai dari 10 – 60 % (Subekti et al. 2005). Meskipun berbagai survei telah dilakukan namun umumnya masih bersifat sporadik dan tidak dilakukan secara terencana. Salah satu syarat untuk dapat memprogram dan melaksanakan diagnosis untuk kepentingan epidemio- logis dengan sigi secara berkala atau periodik adalah ketersediaan perangkat diagnostik di dalam negeri. Demikian pula halnya dalam diagnsosis rutin di laboratorium klinik, umumnya masih tergantung pada produk impor. Ketergantungan pada kit komersial seringkali tidak sesuai untuk tujuan tertentu, misalnya kit ELISA untuk manusia tidak dapat dipakai untuk hewan. Aplikasi diagnosis toksoplasmosis pada