World Applied Sciences Journal 33 (1): 27-32, 2015 ISSN 1818-4952 © IDOSI Publications, 2015 DOI: 10.5829/idosi.wasj.2015.33.01.14570 Corresponding Author: Mona S. Elneklawi, Department of Biomedical Equipments & Systems, Faculty of Applied Medical Sciences, October 6 University, Cairo, Egypt. 27 Evaluation of Extremely Low Frequency Electric Field Role on Mice Induced with Ehrlich Tumor Fadel M. Ali, Reem H. Elgebaly and Mona S. Elneklawi 1 1 2 Department Biophysics, Faculty of Science, Cairo University, Egypt 1 Biomedical Equipments and Systems Department, 2 Faculty of Applied Medical Sciences, October 6 University, Egypt Abstract: In this work the evaluation of extremely low frequency electric field (ELF EF) role on mice induced with Ehrlich tumor was studied by employing 0.5 Hz electric field and 0.7 Hz electric field. Thirty female Balb/c mice 22±2 g carrying Ehrlich tumor in the thigh divided into three equal groups. Group A represents the control, group B was exposed to 0.5 Hz and group C was exposed to 0.7 Hz. Histopathological examination was used to investigate the impact of ELF EF on Ehrlich tumor, liver and spleen in both exposed groups. The results indicated that general health improvement was observed in exposed groups. However, group B showed more improvements than did group C. By comparing data from current and previous work, it was concluded that the use of ELF EF proved to be effective in controlling tumor cells and recovery of other organs such as liver and spleen. Key words: Extremely low frequency Electric field Ehrlich tumor Spleen Histopathological studies Liver INTRODUCTION on physical parameters of the EMF (frequency and The principle that electric fields can provoke and/or the type of bacteria cells used. The biological biological effects has been known since the middle of the effects of EMF have been studied, particularly on cells 19 century. Currently, the biological possessions of (T-lymphocytes, tissue and tumors), biomolecules and th extremely low-frequency electromagnetic field (ELF EMF) microorganisms, as well as on other animals and people have attracted attention of many researchers to not only [6]. More recently, its interaction with cell membranes [7] establish the basic mechanisms of its interaction to living has been studied. Many scientific studies verified that systems but also its potential of practical applications [1]. electric and/or magnetic fields at extremely low frequency The influence of electromagnetic fields (EMF) on (<300 Hz) can influence the biological systems, change biological systems has been attracting interests of more the cellular proliferation [8], stimulate the ATP production and more scientists during the past two decades. A great [9] and change the flow of ions through the membranes deal of research has been conducted and convincing [7], control the growth of Ehrlich tumors in mice [10-13], evidence indicates that exposure to low frequency EMF death of cancer cells [14], fungi [15, 16] and bacterial cells can induce the alterations in living systems [2]. Several [17, 18]. Ehrlich tumor is a species-specific, transplantable studies have been performed to verify direct effects neoplasia from malignant epithelium that corresponds to exerted by ELF EMF on cell functions. Bacteria have also mice’s mammary adenocarcinoma. It grows in several been used in the studies with ELF EMF [3]. In particular, strains of this animal species: in an ascitic form when it has been demonstrated that ELF EMF can negatively [4] inoculated in the peritoneal cavity and in the solid form or positively [5] affect functional parameters (cell growth when subcutaneously inoculated. In order to elucidate and viability) and bacteria antibiotic sensitivity depending factors involved in carcinogenesis, several researchers magnetic flux density) applied, the time of exposure