Research Article
A Multibiomarker Approach to Assess the Health State of Coastal
Ecosystem Receiving Desalination Plants in Agadir Bay, Morocco
Ahmed Elazzaoui , Abdellatif Moukrim, and Latifa Lefrere
Laboratory of Aquatic Systems: Marine and Continental Ecosystems, Department of Biology, Faculty of Sciences,
Ibn Zohr University, BP 8106, Agadir, Morocco
Correspondence should be addressed to Ahmed Elazzaoui; ah.elazzaoui@gmail.com
Received 9 April 2019; Revised 17 September 2019; Accepted 19 November 2019; Published 16 December 2019
Academic Editor: Rizwan Hasan Khan
Copyright © 2019 Ahmed Elazzaoui et al. is is an open access article distributed under the Creative Commons Attribution
License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is
properly cited.
e present study aims to evaluate the initial health status of two stations receiving seawater desalination plants in Agadir Bay
(Tifnit-Douira and Cap Ghir) and to assess their potential environmental impact on the marine ecosystem health. Six pairs of
mussels (Mytilus galloprovincialis) were collected at six sampling sites on a monthly basis over two years. Each pair was
homogenized to obtain the postmitochondrial fractions (S9). Toxicological effects were measured using a multibiomarker
approach based on either acetylcholinesterase (AChE), glutathione S-transferase (GST), catalase (CAT), and malondial-
dehyde (MDA) rate. e results show a seasonal variation of the biomarkers: their activities increase in summer and decrease
in spring and winter. High activities were recorded during summer in Cap Ghir (17.94 ± 0.88; 5.91 ± 052 nmol/min/mg of
protein) for CAT and MDA, respectively. In Tifnit-Douira, low activities were recorded during winter for GST
(3.74 ± 0.52 nmol/min/mg of protein) and during spring for the CAT (3.52 ± 0.45 nmol/min/mg of protein). e fluctuations
in the activities of measured biomarkers could be attributed to different factors including the changes in environmental
parameters, the influence of seasonal variation, and the contamination of the aquatic ecosystem. e data obtained in this
study should be taken into account in the monitoring and management of the health of the ecosystems when the desalination
plants are established.
1. Introduction
e national and regional sectoral strategy makers (Green
Morocco Plan, Plan Azur, Halieutis, etc.) were planned to
realize many development programs and projects in the
Souss-Massa region [1–5]. However, water availability is a
limiting factor for the implementation of these projects and
for any regional development [6, 7], particularly in regions
that are characterized by dry climate conditions and over-
exploitation of water resources (dam and groundwater).
To overcome these problems, the regional council, as a
part of its strategy, has invested in the installation of seawater
desalination plants, in Agadir Ida Outanane (Cap Ghir) and
Chtouka Ait Baha Province (Tifnit-Douira), to produce
water and to meet the requirements of development projects
[8, 9].
Besides the fact that scientific data of Tifnit-Douira and
those of Cap Ghir are not available, the importance of
ecological coastal ecosystems and the potential impact of the
desalination plants prompted us to launch a new study to
assess the initial health status of the ecosystems.
is study was established by using a multidisciplinary
approach to assess the coastal ecosystem health by several
environmental monitoring programs such as Mussel Watch
Program [10–13]. It will be useful to follow the environ-
mental changes of these ecosystems after the implementa-
tion of projects. Our research laboratory has often used this
approach for several years to evaluate and to follow-up the
environmental quality of Agadir Bay ecosystems [14–22]. It
is based on the use of many biomarkers, such as acetyl-
cholinesterase, catalase, glutathione S-transferase, and
malondialdehyde, as pollution indicators of seawater quality
Hindawi
e Scientific World Journal
Volume 2019, Article ID 5875027, 9 pages
https://doi.org/10.1155/2019/5875027