Iraqi Journal of Veterinary Sciences, Vol. 26, Supplement II, 2012 (53-55) Proceedings of the 6 th Scientific Conference, College of Veterinary Medicine, University of Mosul 53 Thin layer chromatographic investigation of some veterinary drugs Z.S. Hamad and B.M. Yahya Department of Pharmacology, College of Pharmacy, University of Mosul, Mosul, Iraq Abstract A simple technique, using solvent extraction and thin layer chromatography for the detection and separation of a number of commonly prescribed veterinary drugs was studied. Blood and urine have been considered as possible biological samples taken from the animals. The extraction of each sample was done according to the type of the drug as it is acidic or basic, then the extract was spotted on TLC plate by capillary tube after lining the starting line first. Separations of the components of the samples were developed after running the plate in a tight lid tank containing solvent (mobile phase). The identification of separated spots were carried out after lining the end line for the solvent front then by spraying the plate with mercurous nitrate reagent for acidic drugs and acidic iodoplatinate or potassium permanganate reagent for basic drugs. The rate of flow value (R f ) for each compound was measured and the color of spots were determined. This work provides a simple and rapid qualitative technique for the detection of veterinary drugs used at therapeutic doses and in overdosage. Keywords: Thin layer chromatography; Veterinary drugs; Drug analysis. Available online at http://www.vetmedmosul.org/ijvs طرية البي الدوية بعض عن التحري بو س ايقة الرق الطبقة إستشراب طة حيى يحمد م باسل و حمد سطام زينة فرع الدوية، الصيدلة، كليةموصل ال جامعةموصل ال، ، العراق خلصة ال إستعماستخلص ال عملية بعد بسيطة طريقة الدراسة ھذه وصفتيقة الرق الطبقة إستشراب طريقة ل لكشف وفصل الدوية مند عد ستعمال اللشائعة اطرية البي. يلتحل ال ولكشف لستعمل تنات الحيوا من تؤخذ عينات افضل الدرار و الدم يعتبر الدويةجود و عن. تم حيث ا علىلعينات ا ھذه وضع وتملقاعدية ا بالدوية خاصة والخرةلحامضية ا بالدوية خاصةھما إحداقتين بطريلعينات ا ھذه استخلص لطبقة الشعريةيبلناب ا باستعماليقة الرق البتداء خط تحديد بعد في وضعت ثم تحويلغطاء احكمة م اواني على) الطور المتحرك( بكللخاص ا الدوية منوعين الن، قصيرة زمنية فترة بعد العينة في الموجودةلمادة ا تنفصل سوف الطبقة اعلى الىولمحل ال تحرك مع خطد يحد و لنتھاء ا المتحركولمحل ال لجريان الدوية من نوع بكللخاصة الكاشفة ايل بالمحاليقة الرق الطبقة وترش البق مكان لتحديد ع ة قيمة تقاس ثم الـR f عينة كل لوند ويحد لھا. طريقة أعطت الدراسة ھذه نوعية وسريعة سھلة لعلجية ال بالجرعطرية البي الدوية منجموعة م عن لكشف المفرطة والجرع. Introduction Animal toxicoses are a frequent presenting complaint in veterinary emergency clinics and general practice (1). Over the years, biological fluid samples have been used for drug analyses (2). Identification of the drug is a prerequisite for optimal treatment (3). The use of thin layer chromatography (TLC) for the separation and identification of compounds is one of the earliest chromatographic techniques (4). It has been increased over the past few years for drug analyses, owing to the short development time of modern plates (5). Less than one hour is usually enough to separate and identify very small amounts of a specific drug. A second advantage is the high reproducibility obtained with this technique using reproducibly coated and conditioned plates with accurate sample application technique (6,7). This work was carried out to investigate the possibility of detection and separation of a number of a commonly