Int.J.Curr.Microbiol.App.Sci (2019) 8(2): 3049-3058 3049 Original Research Article https://doi.org/10.20546/ijcmas.2019.802.357 Analysis of Quinolones Residues in Milk using High Performance Liquid Chromatography Priyanka, Vijay J. Jadhav * , Sneh Lata Chauhan and S.R. Garg Department of Veterinary Public Health & Epidemiology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana- 125004, India *Corresponding author ABSTRACT Introduction Since the early 1960s, there has been two-fold increase in per capita milk consumption of developing countries. This increased demand of milk made it essential to adopt extensive animal husbandry practices. Use of veterinary drugs for taking cure of variety of ailments in farm animals is an integral component of such extensive animal husbandry practices. Antibiotics are the most widely used veterinary drugs for therapeutic and prophylactic purposes and also as growth promoter in dairy animals which may appear in milk as residues for a certain time period (Wassenaar, 2005). They are also be used at sub-therapeutic levels to increase feed efficiency, promote growth and prevent diseases (Ronquillo and Harnandez, 2016). According to one estimate, approximately 80% of the food-producing animals receive medication for part or most of their lives (Pavlov et al., 2008). The use of antibiotics therapy to treat and prevent udder infections in cows is a key component of mastitis control in many countries. The extra-label use International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 8 Number 02 (2019) Journal homepage: http://www.ijcmas.com In the present study, High Performance Liquid Chromatography with Ultra-Voilet detector (HPLC-UV) technique was standardized and validated for the detection and quantitation of quinolones antimicrobial residues viz. enrofloxacin, norfloxacin and ciprofloxacin from milk. The standardization procedure showed that the values for the system precision (% RSD) for both the analytes was 11% for area and <0.9% for retention time), linearity (r 2 >0.98), specificity and accuracy (70-110%) and precision (<10%) were within accepted range and demonstrated system suitability for analysis of milk samples. The standardized and validated method was applied for the detection of quinolones residues from 100 randomly milk samples collected from local market of Hisar (Haryana). Mean concentrations of norfloxacin and enrofloxacin antimicrobial residues in market milk samples were 3.54 and 2.02 μg/kg, respectively. A total of 8 samples were found to be containing quinolone antimicrobial residues. Comparison of antimicrobial concentration in each positive sample of milk with international MRLs showed that, none of the three antimicrobial was responsible for violations of set residue limits. It was concluded that milk is significant source of antimicrobial residues. Keywords HPLC, Quinolones, Milk, Antimicrobial residues, MRL Accepted: 22 January 2019 Available Online: 10 February 2019 Article Info