Plant Archives Journal home page: www.plantarchives.org Stephania glabra is a wild medicinal plant possessing multiple uses as anti-cancer, antimicrobial and antioxidant activities. Diferent extracts and fractions were prepared from S. glabra tubers and were analyzed for diferent activities. Evaluation of the total phenolic and favonoid content revealed that chloroform extract of S. glabra contain maximum phenolic content and maximum favonoid content was found in methanolic extract. Dry powder analysis of the tubers revealed the presence of saponins, alkaloids and proteins. Ethyl acetate extract of S. glabra tubers showed highest antioxidant potential as revealed by ABTS radical scavenging activity. Methanolic extract has the highest anti-cancer activity while chloroform extracts and fractions have highest antimicrobial activity. These activities can be attributed to the high amount of the phytoconstituents present in these extracts Keywords: Anti-cancer; antimicrobial; antioxidant; growth curve; phytochemicals EVALUATION OF ANTIOXIDANT, ANTI-CANCER AND ANTIMICROBIAL ACTIVITY OF DIFFERENT EXTRACTS AND FRACTIONS OF STEPHANIA GLABRA Prachi Vaid 1 , Adesh K. Saini 2 and Reena V. Saini 1, 2* *E-mail: reenavohra10@gmail.com 1 School of Biotechnology, Faculty of Applied sciences and Biotechnology, Shoolini University, Solan, HP, India 2 Department of Biotechnology, MMEC, Maharishi Markandeshwar (Deemed to be University) Mullana-Ambala, India (Date of Receiving-09-11-2020; Date of Acceptance-06-02-2021) ABSTRACT DOI Url: https://doi.org/10.51470/PLANTARCHIVES.2021.v21.no1.081 INTRODUCTION Traditional uses of medicinal plants in the form of powder, liquid or mixtures include as treatment against various ailments in India. These herbal plants have proved pivotal in the development of new drugs or phytomedicines for treatment of diseases. Many plants with medicinal properties have been reported from India (Chopra et al., 1958; Dhar et al., 1965, 1968; Dhawan et al., 1980; Rao and Krishnaiah, 1981; Bhakuni et al., 2002; Shilaskar and Parasher, 1989). Several plant species (medicinal or non- medicinal) have been used for their remedial properties for centuries against human diseases as these contain components of therapeutic value (Iwu et al., 1999). During the last 20 years, various plant parts such as leaves, roots, shoot, rhizomes, etc. or their extracts have been used extensively as complementary and alternative medicine (Rios and Recio, 2005). Antimicrobial potential of diferent medicinal plants is also studied extensively worldwide (Kaur and Arora, 2009). Diseases like cancer, jaundice and asthma have been treated with the plant rhizomes and tuberous roots in indigenous systems of medicines in Asia especially in India, Pakistan and China (Asif, 2012). The rhizomes are the horizontal underground plant stem capable of producing the shoot and root systems of a new plant. The capability of rhizome to produce shoots and roots, allows the parent plant to propagate vegetative and to perennate underground (Auta et al., 2011). The interest of pharmaceutical industries has increased to discover the new drugs from the ethnobotanicals and also to provide new and alternative methods to synthetic drugs for the treatment of dreadful diseases. Stephania glabra belongs to Menispermaceae family, which includes 65 genera and 350 species (Semwal et al., 2010, Hong et al., 2015, Moongkarndi et al., 2004, Nakaoji et al., 1997, Montririttigri et al., 2008 and Yang et al., 2010). S. glabra (Roxb.) Miers, vern., Gindaru is one of the well-known members of this genus. The plants belonging to this family (especially tubers) have wide range of medicinal properties and they have been used traditionally by the locals for the treatment of dysentery, hyperglycemia, asthma, tuberculosis, cancer, fever, intestinal complaints, sleep disturbances and infammation (Kirtikar and Basu., 2004, Gaur., 1999 and Chopra et al., 1958). S. glabra is a large, climbing shrub, indigenous to the lower parts of Indian Himalayan region (Gaur 1999). Large number of phytochemical studies on this plant has led to the isolation of over 30 alkaloids with a very few favonoids, and other constituents (Bhakuni & Gupta 1982; Pal et al., 1995). The present communication deals with the analysis of antioxidant, anti-cancer and antimicrobial activities of S. glabra tuber extracts and fractions. MATERIALS AND METHODS e-ISSN:2581-6063 (online), ISSN:0972-5210 Plant Archives Volume 21, No 1, 2021 pp. 579-591