Clove Oil (Syzygium aromaticum) Edible Film Formulation and Antibacterial Activity Test against Streptococcus mutans Wida Ningsih 1 *, Afdhil Arel 2 1 Universitas Baiturrahmah, Jalan Raya By Pass, Aie Pacah, Koto Tangah, Aie Pacah, Kec. Koto Tangah, Kota Padang, Sumatera Barat 25586 2 Universitas Muhammadiyah Sumatera Barat, Jl. Pasir Jambak No.4, Pasie Nan Tigo, Kec. Koto Tangah, Kota Padang, Sumatera Barat 25172 Abstract Clove oil contains eugenol as an antibacterial. Meanwhile, products containing clove oil have been widely used as toothpaste and mouthwash. In this study, clove oil was formulated in the form of edible film because it is practical, easy to use, and could be used without water like other oral hygiene preparations. The edible film is a thin layer film made of consumable materials used as a carrier of antibacterial compounds. Clove oil edible film was then formulated with clove oil concentrations of 1%, 1.5%, and 2% and determined for its antimicrobial activity against Streptococcus mutans. Clove oil edible film preparations were evaluated under their physical properties, including friability, drying shrinkage, pH, thickness, and swelling ability. Antibacterial activity testing of clove oil edible film was conducted, employing the blood agar diffusion method against Streptococcus mutans. The physical evaluation of the clove oil edible film showed almost the same physical properties as the comparison (GF). Clove oil edible film test results revealed the greatest inhibition at F1 of 18.6 mm ± 0.577, F2 of 22.3 mm ± 2.081, and F3 of 25.3 mm ± 1.527. According to David and Stout, the inhibition activity of bacteria on F3 was categorized as a very strong group inhibition response. In addition, ANOVA test analysis results uncovered that the concentration of clove oil affected the inhibition of the Streptococcus mutans bacteria with a significance value of 0.000 (p <0.05). Also, Duncan's test exhibited that each concentration of clove oil had a significant difference in the inhibition of Streptococcus mutans bacteria. Keywords: antibacterial, edible film, eugenol, agar diffusion, clove oil Data of article Received Reviewed Accepted : : : 28 Apr 2021 23 July 2021 18 Aug 2021 DOI 10.18196/jfaps.v2i1.11640 Type of article: Research INTRODUCTION Clove oil has secondary metabolites in the form of essential oil with the highest eugenol content so that it has biological activity as an antiseptic and analgesia in the treatment of teeth and mouth, * Corresponding author, e-mail: nwida777@gmail.com antifungal, antibacterial, antioxidant, and anticarcinogenic 1 . Clove oil can be isolated from the leaves (1-4%), stems (5-10%), and clove flowers (10-20%). Previous research was about the bactericidal effect of clove oil on Streptococcus mutans and Streptococcus pyogenes bacteria, which