Draft Genome Sequence of a Bovine Enterovirus Isolate Recovered from Sewage in Nigeria T. O. C. Faleye, a,b O. M. Adewumi, a O. A. Olayinka, a E. Donbraye, c B. Oluremi, d U. E. George, a O. A. Arowolo, e E. C. Omoruyi, f M. I. Ifeorah, g A. O. Oyero, h J. A. Adeniji a,h a Department of Virology, College of Medicine, Faculty of Basic Medical Sciences, University of Ibadan, Ibadan, Nigeria b Department of Microbiology, Faculty of Science, Ekiti State University, Ado-Ekiti, Nigeria c Department of Medical Microbiology and Parasitology, Obafemi Awolowo University, Ile-Ife, Nigeria d Department of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Ibadan, Ibadan, Nigeria e Viral Vaccines Production Division, National Veterinary Research Institute, Vom, Plateau State, Nigeria f Institute of Child Health, College of Medicine, University of Ibadan, Ibadan, Nigeria g Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, University of Nigeria, Nsukka, Nigeria h WHO National Polio Laboratory, University of Ibadan, Ibadan, Nigeria ABSTRACT We describe the draft genome of a bovine enterovirus (EV) isolate re- covered from sewage in Nigeria. This isolate replicates on both RD and L20B cell lines but is negative for all EV screens in use by the Global Poliovirus Eradication Ini- tiative (GPEI). It contains 7,368 nucleotides (nt) with 50.2% G+C content and an open reading frame (ORF) with 6,525 nt (2,174 amino acids). E nteroviruses are members of the genus Enterovirus (EV), family Picornaviridae, order Picornavirales. Poliovirus (PV) is the type member of the genus and, courtesy of the Global Poliovirus Eradication Initiative (GPEI), is isolated in about 150 specialized laboratories globally. The laboratories use RD (of human origin) and L20B (engineered mouse cells expressing the poliovirus receptor) cell lines for PV isolation (1, 2). All isolates that grow on both cell lines are assumed to be polioviruses and subsequently subjected to molecular identification (3). Here, we describe the genome of an isolate, EV_NGR_2017, that grew on both cell lines but is not poliovirus. The isolate was recovered from a sewage-contaminated water sample collected in Borno State, Nigeria, in 2017. It was inoculated into (and replicated on) both RD and L20B cell lines but was not poliovirus. The genome of the isolate was extracted using a total RNA extraction kit (Jena Bioscience, Germany) and used for cDNA synthesis as previously described (4). The single-stranded cDNA was shipped to a commercial facility (MR DNA, TX, USA), where library preparation and genome sequencing and assembly were done. Library preparation was done using the TruSeq RNA LT sample preparation kit (Illumina) following the manufacturer’s recommendations. Sequencing was done paired end for 300 cycles using the MiSeq system (Illumina), assembly of the 7.8 million reads was done using Newbler (Roche), and annotation of the Enterovirus E (EV-E) genome was done by aligning it (using MEGA5 software [5]) with previously charac- terized and annotated EV-E genomes in GenBank. Precisely 7,810,328 reads were generated. The draft genome is 7,368 nucleotides (nt) long, with a G+C content of 50.2%, and was assembled from 6,862 (0.09%) reads. The 5= untranslated region (5=-UTR), open reading frame (ORF), and 3=-UTR contain 800 nt, 6,525 nt (2,174 amino acids [aa]), and 43 nt, respectively. The ORF encodes 1 polypro- tein that can be cleaved into 3 (P1 [2,517 nt, 839 aa], P2 [1,737 nt, 579 aa], and P3 [2,271 nt, 756 aa]) and subsequently into the 11 proteins encoded in EV genomes. A BLASTn Received 24 October 2018 Accepted 8 November 2018 Published 13 December 2018 Citation Faleye TOC, Adewumi OM, Olayinka OA, Donbraye E, Oluremi B, George UE, Arowolo OA, Omoruyi EC, Ifeorah MI, Oyero AO, Adeniji JA. 2018. Draft genome sequence of a bovine enterovirus isolate recovered from sewage in Nigeria. Microbiol Resour Announc 7:e01466-18. https://doi.org/10.1128/MRA .01466-18. Editor Irene L. G. Newton, Indiana University, Bloomington Copyright © 2018 Faleye et al. This is an open- access article distributed under the terms of the Creative Commons Attribution 4.0 International license. Address correspondence to O. M. Adewumi, adewumi1@hotmail.com. GENOME SEQUENCES crossm Volume 7 Issue 23 e01466-18 mra.asm.org 1