American-Eurasian J. Agric. & Environ. Sci., 15 (1): 115-121, 2015 ISSN 1818-6769 © IDOSI Publications, 2015 DOI: 10.5829/idosi.aejaes.2015.115.121 Corresponding Author: Habibur Rahman,Anurag Pharmacy College, Kodad, Telengana State, India. 115 In-vitro Anti-inflammatory and Anti-arthritic Activity of Oryza sativa Var. Joha Rice (An Aromatic Indigenous Rice of Assam) Habibur Rahman, M. Chinna Eswaraiah and A.M. Dutta 1,2 1 2 Anurag Pharmacy College, Kodad, Telengana State, India 1 Assam Down Town University, Guwahati, Assam, India 2 Abstract: The objective of present work is to study the in-vitro anti-inflammatory and anti-arthritic activity of Joha Rice, an aromatic indigenous rice of Assam, India. The ethanolic extract of Oryza sativa Var. Joha rice (EEOS-JR) was studied for in-vitro anti-inflammatory activity by human red blood cell (HRBC) membrane stabilization method and In-Vitro anti-arthritic activity by bovine serum protein denaturation method and egg albumin denaturation method. The activity of ethanolic extract of Joha rice was compared with standard anti- inflammatory drug Diclofenec. In our result it is found that EEOS-JR at concentration of 100, 250 and 500 mcg/ml showed 51.12, 58. 75 and 63.77% protection of HRBC in hypotonic solution respectably, whereas, standard diclofenac at 50, 100 and 250 mcg/ml which showed 68.11, 73.83 and 76.17% protection of HRBC in hypotonic solution respectably. It also showed 39.29%, 52. 78% and 60.47% inhibition of denaturation @ 100, 250 and 500 mcg/ml of bovin serum whereas, standard diclofenac @ 100, 250 and 500 mcg/ml showed 93.20, 95.41 and 96.91% inhibition of denaturation of bovin serum. In egg albumin denaturation method at concentration of 100, 250 and 500 mcg/ml showed 75.00, 80.31 and 84.15% inhibition of egg albumin denaturation whereas, standard diclofenac @100, 250 and 500 mcg/ml which showed 27.78, 45.84 and 69.77% inhibition of egg albumin denaturation. It was found that ethanolic extract of Joha rice was more potent in inhibition of egg albumin denaturation than diclofenac. Finally, from results it can be concluded that Oryza sativa Var. Joha rice; an indigenous aromatic rice of Assam posses good in-vitro anti-inflammatory and anti-arthritic activities. By further extensive research, we can explore the medicinal value of joha rice. Key words: Joha Rice Aromatic Rice In-vitro methods Human red blood cell Anti-inflammatory Anti-arthritic activity INTRODUCTION adequate facilities are playing important hurdles to save Assam is a land of thousands of natural herbs and scientifically evident medicinal drug in present market. medicinal plants. The unique geographical location, Joha Rice is one of the 40,000 varieties of species Oryza abundant of fertile soil, friendly climate and high rain fall sativa and it is popular for its great aroma and equally make a gift of herbal resources. With its vast hills and remarkable taste. It is commonly used to prepare Khiir or forests, Assam is the home to a variety of medicinal Payash or Pulao like traditional recipe [2]. plants such as word famous Tea (Camelia sinensis), Some literature available which mentioned the anti- Sarpagandha (Rauvolfia serpentine Benth.ex.Kur), Pippali oxidant properties of rice. Laokuldilok et al. reported that (Piper longam Linn), Amlakhi (Emblica officinalis several pigmented rice brans have free radical scavenging Gaertn), Hilikha (Terinalia chebula Retz.), Bhomora and antioxidant activity [3]. Muntana and Prasong (Terminalia belerica) etc. More than 300 medicinal plants reported total phenolic contents and their antioxidant have been identified in Assam but only about 5-10% of activities of Thai white, red and black rice bran extracts the plants and herbs are currently utilized [1] and the rest [4]. Rao et al. reported antioxidant and antiproliferative hold a vast potential. Lack of knowledge, space and activities of methanolic extracts from Njavara rice bran [5]. the unique gift of nature in Assam for making