Journal of Biotechnology Research Center Vol. 11 No.2 2017 10 New Record of Penicillium vanoranjei in Iraq العراق فيPenicillium vanoranjei تسجيل جديد ل لفطر * Samarai - Al F. Ghassan * Dheeb . I Batol Hmoody M. Abdulhanmed. mansour - Wafaa khalaf Al College of Education/ Samarra University . *College of Applaied Science/ Samarra University ف المنصور وفاء خل حموديحمديد م عبد الحم* تول عمران ذيب ب غسان فمرائىلسارس ا ا ية التربية كل/ مراء جامعه سا * وم التطبيقيةعلية ال كل/ جمراء امعة سا E-mail address: batoolomran@yahoo.com Abstract The present study aimed to identification of P. vanoranjei based on microscopical and cultural method as a new record isolate in Iraq. Penicillium vanoranjei is a new species in Iraq that produce an orang production pigment after compelet growth on the culure medium. Location, the spesmen collected from women patient admitted to Al Jimhory Hospital in Samaraa city and lived in Al Jilam township during December/ /2016 in Salah Al Deen Governorate- Samaraa City. Morphological and microscopichal charecterstic appeared that the top view of the culture slightly raised in the center; mycelia was white near the margins, the reversed view appeared Dark brown. Microscopic examination illustrates that the Conidiophores were monoverticillate (unbranched) Penicillium vanoranjei . ملخص ال نوعلفطر في العراق لول مرة لديد سجل جPenicillium vanoranjei حصلية بعد الت علة في ال والتنمي صبيغ وسط الزرعي من لنساء اجلم منطقة ال يسكنلواتي والح الدين,مراء/ صل مدينة سالعام فيمراء اتشفى سات الى مسجعا المرا المرضىفي الري ة و يعان ي ن م ن ن اولل شهر كانوصابة فطرية خل ا- 2016 يل ع قلنمو بشكلع الرتفاشخصية اظهرت ا المظهرية والتلصفات . ا ن فطريةركز مع خيوط د الم الكونيلمجهري بان اظهر التشخيص المستعمرة. كماوما لي الداكن عم البنلونرة مع الفة المستعمالقرب من حا بيضاء ب دات ال سبورية لفطر ومطابقة لة غير متفرعةحادي اPenicillium vanoranjei . مفتاحية: كلمات, Penicillium vanoranjei ل جديد, تسجي, Penicillium لبرتقالي ا, ختبار زرعي ا Introduction Penicillium vanoranjei is relatively rare species isolated for the first time in Tunisia the maine charecterized by cononies dominated by bright orang sclerotia and production of orange colonies in culture. The history of the name (Dutch = oranje) related to Willem-Alexander Claus George Ferdinand, ‘Zijne Koninklijke Hoogheid de Prins va n Oranje’ (translated from Dutch as: ‘His Royal Highness the Prince of Orange’) and his family, to coincide with his coronation also the name of this fungus when isolated from Tunisia soli was named as a tribute to the Dutch royal family, specifically His Royal Highness the Prince of Orange which consider the first isolation in the world. In May 2014, the International Institute for Species study listed P. vanoranjei as one of the "Top 10 News Species" named in 2013. '''Penicillium vanoranjei C.M. Visagie, J. Houbraken & R.A. Samson, Persoonia 31: 46 (2013) [MB#803782] [1]. Materials And Methods Sample Colection During the period of December/2016 total of 100 scraped samples were collected from patients (18 to 66 years old of both genera) suspected to suffer from dermatophytosis disease, (as clinically identified by a physician) from the Dermatology unit of Al Jimhory Hospital in Samaraa city. The samples were subjected for direct examination using 10% KOH specimen were placed on a microscopic slide, with the few drops of 10% KOH, a cover slip added and warmed over a small flame just before boiling. The slide was examined under the low power and high dry objectives to detect the presence of the fungi and their hyphae[1]. Scraping of each samples culturing on the sabouraud dextrose agar SDA supplemented with 0.04 mg/ml chloramphenicol to inhibit the growth of bacteria , Potato Dextrose agar (PDA), Czapex-dox medium medium, and Malt extract Agar MEA then incubated at 30 °C and examined for 7-14 days [2,3,4,5,6]. A small piece of transparent-adhered tape was touched to the surface of the suspected colony, and then adhered