NJM Vol 7, No 2, 2022 E-ISSN 2686-2565 27 Pengaruh Pemberian Ekstrak Alpukat terhadap Kadar Kolesterol Total Tikus Wistar Jantan dengan Obesitas Kristian David Pasaribu 1 , Runggu Retno Justiani Napitupulu 2 , Jenny Novina Sitepu 3 1 Mahasiswa Fakultas Kedokteran Universitas HKBP Nommensen Medan 2 Departemen Biokimia Fakultas Kedokteran Universitas HKBP Nommensen Medan 3 Departemen Anatomi Fakultas Kedokteran Universitas HKBP Nommensen Medan Korespondensi: Kristian David Pasaribu, Email: kristiandavidpasaribu12@gmail.com Abstract Background: Obesity is a condition where fat accumulates excessively in the body which is along with high levels of total blood cholesterol. Avocados (Persea americana) contains omega-9 and vitamins that can reduce total blood cholesterol levels. Objective: This study aimed to see the effect of avocado extract to reduce total blood cholesterol levels on obese male Wistar rat. Methods: This study used a true experimental pretest-posttest with control group design. Subjects were 32 obese male Wistar rats according to Lee's criteria (BMI> 0,3). Subjects were divided into treatment groups and control groups. Pretest of total cholesterol levels was done before treatment while posttest was done after treatment. The treatment group was given avocado extract, while the control group was not. The data was analyzed by paired and unpaired T tests to see the differences of total cholesterol levels on both treatment and control groups. Results: The results was found that the average of treatment group BMI was 0.4763 and the control group BMI was 0.4831. The average of cholesterol total level of treatment group pretest was 104.58 mg/dL and posttest was 85 mg/dL. Then the control group pretest was 101.68 mg/dL and posttest was 98.56 mg/dL. On the treatment group found a significant differences of pretest and posttest average of total cholesterol levels with value of p = 0.000 (p <0.05). A differences of posttest average of total cholesterol level on the treatment group was lower than the control group, with a value of p < 0.001 (p <0.05). Conclusion: Gave the avocado extract reduce total blood cholesterol levels in obese male Wistar rats. Keywords: avocado (Persea americana), total cholesterol, wistar rats, obesity. Abstrak Latar belakang: Obesitas adalah keadaan dimana lemak terakumulasi berlebihan dalam tubuh yang sering disertai tingginya kadar kolesterol total darah. Buah alpukat (Persea americana) mengandung omega-9 dan vitamin yang dapat menurunkan kadar kolesterol total darah. Tujuan: Melihat pengaruh pemberian ekstrak alpukat terhadap penurunan kadar kolesterol total darah pada tikus Wistar jantan dengan obesitas. Metode: Penelitian ini menggunakan desain true experimental pretest-posttest with control group. Subjek penelitian adalah 32 ekor tikus Wistar jantan dengan obesitas yang sudah memenuhi kriteria Lee ( IMT > 0,3). Subjek dibagi menjadi kelompok perlakuan dan kelompok kontrol. Pemeriksaan kadar kolesterol total pretest dilakukan sebelum perlakuan sedangkan posttest dilakukan setelah perlakuan. Kelompok perlakuan diberikan RESEARCH ARTICLE