Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2016, 8(12):243-247 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 243 Phytochemical Analysis of the Methanol Extract from Leaves of Cassia fistula L Laxman V Gavali, Vitthal S Shivankar, Yashwant A Gaikwad, Amit Surve Tejas Patil, Vaibhav Mali and Gurumeet C Wadhawa * Post Graduate Department of Chemistry, Karmaveer Bhaurao Patil College Vashi, Navi Mumbai, Maharashtra, India _____________________________________________________________________________ ABSTRACT Our aim of study is to investigate the presence of various plant chemicals like salts steroids, triterpenes, alkaloids, saponins, tannins, anthraquinones, flavonoids and iso flavonoid compounds or their derivatives with their biomedical applications .Plant used for study are traditionally known we carry out phytochemical study of the dried leaf of the plant, to extract and fractionate the leaf and thin layer chromatography of the different fractions to study different components. In the phytochemical investigation of the plant, the powdered leaves was gives tests positive for steroids, triterpenes, alkaloids, sap, tannins, anthraquinones and flavonoids. In Navi Mumbai, Panvel region theses leaves are used on wound healing activity like tridex procubace and gulmohar tree. Keywords: Flavonoids; Alkaloids; Materials; Tests; Phytochemical; Chromatography; Tannins; Extraction; Colour _____________________________________________________________________________ MATERIALS AND METHODS Collection and preparation of plant material Plant leaves are used for the study of plant material collected from panvel region of raigad district indentified by Dr.B.K.Auti of RKMM College Ahmednagar and herbarium is preserved in Rayat shikshan Sansthsa Karmaveer Bhaurao Patil college Vashi as voucher no (001) The leaves were dried at room temperature for 13 days and when properly dried the leaves were powdered using clean pestle and mortar, and the powdered plant was size reduced with a sieve. The fine powder was then packed in airtight container to avoid the effect of humidity and then stored at room temperature. Cassia fistula is a medium sized deciduous tree, 10 m tall with a straight trunk to 5 m, 1 m diameter and spreading branches. Stem bark pale grey, smooth and slender when young and dark brown and rough when old. Leaves alternate, pinnate, 30-40 cm long, with 4-8 pairs of ovate leaflets, 7.5-15 cm long, 2-5 cm broad, entire, the petiolules 2-6 mm long. Flowers bright yellow in terminal, drooping racemes, 30-60 cm long; calyx oblong, obtuse, pubescent; corolla with five subequal, obovate, shortly clawed petals, to 3.5 cm across; stamens 10, upper three with erect filaments to 0.7 cm long and with basifixed anthers; lower three curved and filaments with dorsifixed anthers and the median four stamens with erect filaments, to 1 cm long and with versatile, curved anthers; pistil sessile or stalked, ovary pubescent, style to 0.5 cm long and with terminal stigma.Fruit an indehiscent pod, 40-60 cm long by 1-2 cm diameter, cylindrical, pendulous and terete, containing 25-100 seeds. The pod develops numerous transverse septa between the seeds. When fresh the pods contain a black pulp which on drying adheres to the septThe drug "C. fistula", a mild laxative is obtained from the sweetish pulp around the seed. Reported to be laxative, aperient, astringent, purgative, and vermifuge, Indian laburnum is a folk remedy for burns, cancer, constipation, convulsions, delirium, diarrhoea, dysuria, epilepsy, gravel, hematuria, pimples, and glandular tumors. Ayurvedic medicine recognizes the seed as antibilious, aperitif, carminative, and laxative, the root is used for adenopathy, burning sensations, leprosy, skin diseases, syphilis, and tubercular glands, the leaves for erysipelas, malaria, rheumatism, and ulcers, the buds for biliousness, constipation, fever, leprosy, and skin disease, the fruit for abdominal pain, constipation, fever, heart disease, and leprosy. Yunani use the leaves for inflammation, the flowers for a purgative, the fruit as anti-inflammatory,