Research Article In Vitro and In Vivo Anti-Salmonella Evaluation of Pectin Extracts and Hydrolysates from (Cas Mango) (Spondias dulcis) Denis Zofou , 1,2 Golda Lum Shu, 1,2 Josepha Foba-Tendo , 3 Merveille Octavie Tabouguia, 1,4 and Jules-Clement N. Assob 1,5 1 Medical Research and Applied Biochemistry Laboratory (Drug Discovery and Development Research Unit), University of Buea, Cameroon 2 Department of Biochemistry and Molecular Biology, Faculty of Science, University of Buea, Cameroon 3 Department of Chemistry, Faculty of Science, University of Buea, Cameroon 4 Department of Biochemistry, Faculty of Science, University of Yaounde 1, Cameroon 5 Faculty of Health Sciences, University of Buea, Cameroon Correspondence should be addressed to Denis Zofou; zofden@yahoo.com and Josepha Foba-Tendo; jnfoba@gmail.com Received 24 November 2018; Accepted 13 March 2019; Published 23 April 2019 Academic Editor: Letizia Angiolella Copyright © 2019 Denis Zofou et al. Tis is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Background. Te threat to human health posed by multidrug-resistant strains of Salmonella typhi (S. typhi) and Salmonella paratyphi (S. paratyphi) is of growing concern. Generally, there has been increasing resistance and even multidrug resistance to almost all classes of antibiotics. Tis has rendered treatment with antibiotics difcult and costly. Te present study investigated the bioactivity of pectin and pectin hydrolysates derived from a local fruit, Spondias dulcis, against four strains of Salmonellae. Methods. Pectin was extracted from alcohol extractives-free peel by acidic hydrolysis at a temperature of 80 ∘ C for one hour at pH 2 and 4. Te pectin was precipitated with 95% alcohol at an extract to alcohol ratio of 1:10 v/v. Antimicrobial activity was determined using agar well difusion technique. Te minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values were determined using the broth dilution technique. An in vivo study was then carried out with the bioactive extracts against the most resistant bacteria strain, to fully establish the therapeutic efect of these extracts. Balb/C mice were used, and ciprofoxacin was the positive control antibiotic. Te extracts were administered to mice at two doses, 5mg/Kg and 10mg/Kg. Te efcacy of extracts in the treatment of typhoid was evaluated based on survival rate, change in body weight, and change in bacteria load. Results. Only one of the extracts (crude pectin pH 2.5) was active against all the Salmonellae by well difusion, and the growth inhibition varied from 12mm to 15mm at100 g/ml. Tree of the extracts (crude pectin pH 2.5, pH 4, 12h hydrolysate, and pH 4, 1h hydrolysate) had MIC and MBC against all four Salmonellae strains with MIC ranging from 5.68 to 44.45 g/ml and MBC from 11.36 to 44.45 g/mL. Tree treatments, namely, the pH4-12 hr, hydrolysate at 10mg/Kg and 5mg/Kg, and the pH4-1hr, hydrolysate at 10mg/Kg, had therapeutic efects against Salmonella infection in mice. Conclusion. Te present study highlights the potential of pectin oligosaccharides as new source of anti-Salmonella drugs. Further investigations including exploration of mechanism of action of the most active pectin extracts/hydrolysates are envisaged. 1. Introduction Typhoid fever is a systemic prolonged febrile illness caused by certain Salmonella serotypes. Serovars Typhi and Paratyphi of this serotype, Salmonella enteric subspecies enterica, are the etiologic agents that cause typhoid and paratyphoid fevers, respectively. Typhoid fever is encountered worldwide but primarily prevalent in developing countries. Te risk factors for typhoid are similar to those of cholera and other epidemic prone diarrheal diseases and are mainly related to access to safe water and the functionality of sanitation systems as well as food safety. It commonly presents with a sudden onset of fever, headache, abdominal pain, and diarrhea and can quickly progress to a variety of potentially fatal complications, including gastrointestinal haemorrhage and intestinal perforation. Even though the infection is ofen Hindawi Evidence-Based Complementary and Alternative Medicine Volume 2019, Article ID 3578402, 10 pages https://doi.org/10.1155/2019/3578402