1 Supporting Information Synthesis and comparison of linear and hyperbranched multivalent glycosides for C-type lectin binding Pallavi Kiran a , Shalini Kumari a , Jens Dernedde b , Rainer Haag, Sumati Bhatia a, * a Institut für Chemie und Biochemie, Freie Universität Berlin, Takustr. 3, 14195 Berlin, Germany. b Charité- Universitäts medizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health; Institute of Laboratory Medicine, Clinical Chemistry and Pathobiochemistry, CVK Augustenburger Platz 1, 13353 Berlin, Germany *Corresponding author Sumati Bhatia: sumati@zedat.fu-berlin.de Table of contents 1. Material and methods 2. Binding assay by microscale thermophoresis (MST) 3. Binding assay by surface plasmon resonance (SPR) 4. Synthesis and characterization of all intermediates and final compounds 5. 1 H NMR spectra of all the intermediates and final molecules. 6. SPR binding isotherms derived from single-cycle kinetic measurements. 7. GPC data of linear and hyperbranched polyglycerols and 1 H spectra of hyperbranched polyglycerols 8. Structure of hPG10SA0.65. Electronic Supplementary Material (ESI) for New Journal of Chemistry. This journal is © The Royal Society of Chemistry and the Centre National de la Recherche Scientifique 2019