Int.J.Curr.Microbiol.App.Sci (2017) 6(6): 2707-2713 2707 Original Research Article https://doi.org/10.20546/ijcmas.2017.606.322 Isolation and Characterization of Microorganisms from Agriculture Soil of Magnifera indica Orchard Y. C. Rashmi 1* , R. Reshmi 1 , R. Poornima 2 and Sujeet Kumar 2 1 Mount Carmel College, Palace Road Bangalore, India 2 Department of Plant Biotechnology, University of Agricultural Sciences, GKVK, Bengaluru-560065, India *Corresponding author ABSTRACT Introduction Mango (Mangifera indica L.) is an important fruit crop of the tropical and subtropical countries (Litz, 2009). The mango tree is considered to have evolved in the rainforests of South and South-east Asia (Knight, 1980; Krishna and Singh, 2007). India is the largest producer of mango in the world, contributing to nearly 46% of the total world production. The major constrain of mango production is many devastating diseases (Lim and Khoo, 1985; Iqbal et al., 2006; Rajput and Rao, 2007). A range of microorganisms are involved in these diseases such as fungi, algae and bacteria (Litz, 2009). These microbes cause sets of symptoms including dieback, spots, necrosis, mildew scab, blotch, anthracnose and rots in mango trees (Ploetz, 2003; Freeman et al., 1999; Haggag and Abd El- Wahab, 2009). Pseudomonas syringae and Xanthomonas sp. (causing apical necrosis and bacterial black spot respectively) are among the few known bacterial pathogens of mango International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 6 (2017) pp. 2707-2713 Journal homepage: http://www.ijcmas.com A broad range of microorganisms are present in soil of mango orchard which involved in various mango plant diseases. In order to preliminary study for plant pathogenesis the soil samples were collected from GKVK, University of Agricultural Science, Bangalore, Karnataka, India. A number of bacterial and fungal isolates were obtained from soil sample. The bacterial isolates were characterized by Gram staining, Catalase test, MR test, VP test, IND test and Citrate test and fungal isolates were characterized by staining. These analyses revealed the presence of various bacterial pathogen including Klebsiella pneumoniae, Enterobacter aerogenes, Shigella species, Bacillus anthracis, Bacillus subtilis, Staphylococcus species, Streptococcus species, Corynebacterium, Micrococcus, Azomonas species and Rhizobium species. Identification of Aspergillus niger, Aspergillus flavus, Fusarium oxysporum, Penicillium and Rhizopus species characterized as a fungal pathogen. The present study provided baseline information regarding the phytopathogenic bacteria and fungus which associated with soil of mango orchard. Keywords Mango, Bacterial pathogen, Fungal pathogen, Biochemical test. Accepted: 26 May 2017 Available Online: 10 June 2017 Article Info