Rev. Bras. Parasitol. Vet., 13, 4, 143-149 (2004) (Brazil. J. Vet. Parasitol.) RESUMO Para avaliação da mastite em vacas pertencentes a vinte pro- priedades leiteiras de municípios da microrregião do Vale do Paraíba Fluminense-RJ, foi realizado o “California Mastitis Test” em 188 vacas. Após exame, coletaram-se amostras de leite das vacas positivas e espécimes de Stomoxys calcitrans (L.) para realização de isolamento e identificação bacteriana. Foram isoladas 36 cepas bacterianas das amostras de leite, sendo verificadas as seguintes bactérias: Staphylococcus spp. coagulase (+) (27,80%), E. coli (19,50%), S. hyicus (11,11%), Bacillus spp. (8,20%), S. saprophyticus (5,55%), Streptococcus spp. (5,55%), S. aureus (5,55%), S. epidermidis (2,79%), Enterobacter agglomerans (2,79%), Shigella spp. (2,79%), S. intermedius (2,79%), Arcanobacterium pyogenes (2,79%), AVALIAÇÃO DA CAPACIDADE DE Stomoxys calcitrans (LINNAEUS, 1758) EM CARREAR BACTÉRIAS ENVOLVIDAS NAS ETIOLOGIAS DAS MASTITES DE MUNICÍPIOS DO RIO DE JANEIRO ANA PAULA R. MORAES 1 ; PABLO V. BADINI 1 ; MILIANE M. SOARES DE SOUZA 2 ; AVELINO J. BITTENCOURT 3 ABSTRACT:- MORAES, A.P.R.; BADINI, P.V.; SOUZA, M.M.S. DE; BITTENCOURT, A.J. [ Evaluation of Stomoxys calcitrans (Linnaeus, 1758) ability to carry bacteria involved in the etiology of mastitis in counties of Rio de Janeiro.] Avaliação da capacidade de Stomoxys calcitrans (Linnaeus, 1758) em carrear bactérias envolvidas nas etiologias das mastites de municípios do Rio de Janeiro. Revista Brasileira de Parasitologia Veterinária, v. 13, n. 4, p. 143-149, 2004. Universidade Federal Rural do Rio de Janeiro, Instituto de Veterinária, Departamento de Medicina e Cirurgia Veterinária, BR 465 Km 07, Seropédica, RJ 23890-000, Brazil. E-mail: bittenc@ufrrj.br California Mastitis Test was performed in order to evaluate mastitis occurrence in twenty dairy cow properties situated in counties of the Vale do Paraíba Fluminense-RJ. A total of 188 cows were submitted to this test. After, positive samples of milk and Stomoxys calcitrans (L.) specimens were collected in order to perform bacterial isolation and identification. 36 bacterial isolates were obtained from milk samples, and the following bacterias were identified: Coagulase positive staphylococci (27,80%), E. coli (19,50%), Staphylococcus hyicus (11,11%), Bacillus spp. (8,20%), S. saprophyticus (5,55%), Streptococcus spp. (5,55%), S. aureus (5,55%), S. epidermidis (2,79%), Enterobacter agglomerans (2,79%), Shigella spp. (2,79%), S. intermedius (2,79%), Arcanobacterium pyogenes (2,79%) and Enterobacter spp. (2,79%). 43 strains were isolated from flies macerates, detecting Shigella spp. (14,00%), Coagulase negative staphylococci (11,62%), E. agglomerans (9,30%), Enterobacter spp. (9,30%), S. saprophyticus (6,98%), Salmonella typhi (6,98%), S. aureus (6,98%), E. coli (6,98%), Salmonella spp. (6,98%), S. schleiferi ssp. coagulans (2,32%), Coagulase positive staphylococci (2,32%), S. pullorum (2,32%), Proteus vulgaris (2,32%), Shigella flexneri (2,32%), Corynebacterium spp. (2,32%), Bacillus brevis (2,32%), Arthrobacter agilis (2,32%) and Bacillus spp. (2,32%). In all properties it was possible to find animals positive for mastitis, and also the presence of S. calcitrans. The raise in the incidence of mastitis could be related to the presence of the stable fly, once it was possible to observe coincidence in S. aureus isolates from milk and fly samples in two properties, and E. coli, in another one. Another bacterial species such as: Staphylococcus spp. coagulase (+), Bacillus spp., S. saprophyticus, Enterobacter agglomerans, Enterobacter spp. and Shigella spp. were identified from samples of both material collected from different properties, although no coincidence was showed in the isolation. KEY WORDS: Stomoxys calcitrans, stable fly, bovine, bacteria, mastits. 1 Curso de Medicina Veterinária, Universidade Federal Rural do Rio de Janeiro (UFRRJ), BR 465 Km 07, Seropédica, RJ 23890-000. Bolsis- ta CNPq/PIBIC/UFRRJ. 2 UFRRJ, Instituto de Veterinária (IV), Departamento de Microbiolo- gia e Imunologia Veterinária. 3 UFRRJ, IV, Departamento de Medicina e Cirurgia Veterinária. E- mail: bittenc@ufrrj.br