18 | wileyonlinelibrary.com/journal/jph Journal of Phytopathology. 2018;166:18–27. © 2017 Blackwell Verlag GmbH Received: 13 April 2017 | Accepted: 23 August 2017 DOI: 10.1111/jph.12655 ORIGINAL ARTICLE Seedling and adult-plant stage resistance of a world collection of barley genotypes to stripe rust Sanjaya Gyawali 1 | Ramesh Pal Singh Verma 1 | Subodh Kumar 2 | Subhash Chand Bhardwaj 2 | Om Prakash Gangwar 2 | Rajan Selvakumar 2 | Pradip Singh Shekhawat 3 | Sajid Rehman 1 | Dipak Sharma-Poudyal 4 1 Biodiversity and Integrated Gene management (BIGM), ICARDA, Rabat, Morocco 2 Indian Institute of Wheat and Barley Research (IIW&BR), Indian Council of Agricultural Research (ICAR), Shimla, India 3 Rajasthan Agricultural Research Institute (RARI), S.K.N. Agriculture University, Durgapura, Rajasthan, India 4 Oregon Department of Agriculture, Salem, OR, USA Correspondence S. Gyawali, BIGMP, ICARDA, Rabat, Morocco. Email: gyawalisanjaya@gmail.com Abstract Barley stripe rust caused by Puccinia striiformis f.sp. hordei (PSH) is one of the major diseases in barley production regions worldwide. A total of 336 barley genotypes with diverse genetic backgrounds targeted for low-input barley production were tested for seedling and adult-plant stage resistance against six PSH races (0S0, 0S0-1, 1S0, 4S0, 5S0 and 7S0) originated from India. The seedling resistance was evaluated by inoculat- ing the barley genotypes with six races separately under controlled conditions in Shimla, India. The same barley genotypes were evaluated for adult-plant stage resist- ance in the Agricultural Research Station (ARS) of Rajasthan Agriculture University, Durgapura, Rajasthan, India. Out of the 336 barley genotypes tested for seedling re- sistance, 119 (35.4%), 101 (30.1%), 87 (25.9%), 100 (29.8%), 91 (27.1%) and 70 (20.8%) genotypes were resistant to races 0S0, 0S0-1, 1S0, 4S0, 5S0 and 7S0, respectively. In the field, 102 (30.3%) genotypes showed the resistance response of which 18 (5.3%) genotypes were highly resistant to PSH. Barley genotypes AM-14, AM-177, AM-37, AM-120, AM-300, AM-36, AM-103, AM-189, AM-291, AM-275 and AM-274 showed resistance response to all six races at seedling and adult-plant stages. Seedling resist- ance reported in the current study is effective against the newly emerged race 7S0 and previously reported five races in India. Therefore, resistant barley genotypes iden- tified in the current study provided effective protection against all six races at seedling and adult-plant stages. The stripe rust resistance identified in the current studies may be potential donors of stripe rust resistance to barley breeding programmes in India and elsewhere. KEYWORDS barley, race, resistance, stripe rust, yellow rust 1 | INTRODUCTION Barley (Hordeum vulagere L.) is one of the most important cereal crops grown on more than 49.7 million ha (FAOStat 2015) and is mainly used as feed, food and malt in many countries (Newman & Newman, 2006). This cereal is adapted to dry areas characterized by erratic rain and poor soil fertility which are often described as low-input barley (LIB) production systems. Biotic stresses, mainly stripe rust (caused by Puccinia striiformis Westend. f.sp. hordei Erikss.) (PSH), cause significant yield losses in barley. Stripe rust often causes serious epidemics in South Asia (India, Nepal and Pakistan) (Bahl & Bakshi, 1963; Bakshi, Bahl, & Kohli, 1964; Luthra