~ 202 ~  The Pharma Innovation Journal 2018; 7(11): 202-204 ISSN (E): 2277- 7695 ISSN (P): 2349-8242 NAAS Rating: 5.03 TPI 2018; 7(11): 202-204 © 2018 TPI www.thepharmajournal.com Received: 13-09-2018 Accepted: 14-10-2018 Parveen Kumar Veterinary Surgeon, Department of Animal Husbandry, Haryana, India Anand Kumar Pandey Department of Veterinary Clinical Complex, LUVAS, Hisar, Haryana, India Ajit Verma Department of Veterinary Gynaecology and Obstetrics, LUVAS, Hisar, Haryana, India Sandeep Kumar Department of Veterinary Gynaecology and Obstetrics, LUVAS, Hisar, Haryana, India SK Phulia ICAR- Central Institute for Research on Buffalo, Hisar, Haryana, India Sandeep Kumar Department of Veterinary Physiology and Biochemistry, LUVAS, Hisar, Haryana, India RK Sharma ICAR- Central Institute for Research on Buffalo, Hisar, Haryana, India Correspondence Parveen Kumar Veterinary Surgeon, Department of Animal Husbandry, Haryana, India Evaluation of effect of different estrus synchronization protocol on oxidative stress during summer season in cyclic Murrah Buffaloes Parveen Kumar, Anand Kumar Pandey, Ajit Verma, Sandeep Kumar, SK Phulia, Sandeep Kumar and RK Sharma Abstract The present study was conducted with the objective to compare the estrus synchronization protocols on oxidative stress parameters in cyclic Murrah buffalo during summer season. Forty cyclic Murrah buffaloes were synchronized for estrus with standard Ovsynch (n=20) and Doublesynch protocol (n=20) and inseminated fixed time at 8 and 24hr of last GnRH injection. Blood samples were analyzed for the level of oxidative stress parameters. Overall, there was no significant difference (P<0.05) found between Ovsynch and Doublesynch treated buffaloes on the day of start of protocol and day of AI (Artificial Insemination) in overall MDA, GSH-Px and SOD concentration. Keywords: Summer, Murrah buffaloes, Oxidative stress Introduction The physiological and environmental stressors arising from high milk production within intensive management systems impair reproductive performance in high yielding buffaloes. Oxidative stress occurs due to an imbalance between the systemic manifestations of reactive oxygen species (ROS) and a biological system's ability to readily detoxify the reactive intermediates or to repair the resulting damage [2] . ROS are also implicated in the reproductive functions such as ovarian steroidogenesis, oocyte maturation, corpus luteal function and luteolysis, thus being related to female fertility [3] . Due to harsh and dry weather during summer season enhance stress for the continuation of reproductive activity in buffalo, probably; an increase in summer-induced oxidative stress is one of the reasons and which estrus synchronization protocol is better in relation with oxidative stress. Therefore, the present study was planned to compare the oxidative stress in buffalo subjected to Ovsynch and Doublesynch estrus synchronization during summer season. Material and Method The study was conducted on 40 cyclic Murrah buffaloes subjected to estrus synchronization via Ovsynch [5] and Doublesynch protocol for estrus synchronization [11] . Buffaloes were divided into two groups as listed 20 in each (having parity:- 1-5; body weight:- 400-600 kg and body condition score:- 3-5) maintained at two locations: (1) Dairy Farm of Central Institute for Research on Buffaloes (CIRB), Hisar and, (2) at Field, Fatehabad district. The blood samples were collected on day of beginning of the protocol and on the day of AI for assessing oxidative parameters viz. Malondialdehyde (MDA), Glutathione peroxidase (GSH- Px) and Superoxide dismutase (SOD) per ml of hemolysate. The procedure for hemolysate preparation involved separation of blood plasma, washing of erythrocytes three times with normal saline solution followed by centrifugation (1500g x 10 min). Thereafter, supernatant decanted and chilled distilled water was added slowly to erythrocyte pellet with constant stirring up to the level of initial blood volume. The hemolysate were stored at -20*C until analysis. Lipid peroxidation was evaluated in terms of MDA formed by using thiobarbituric acid-reactive substances (TBARS; [13] ). Also, the activity of GSH-Px [6] and SOD [9] in erythrocyte lysate was assayed. The student’s T test was employed to compare the differences between the oxidative stress parameters between the day of start of study and day of AI in both groups.