~ 133 ~ Journal of Entomology and Zoology Studies 2020; SP-8(2): 133-137 E-ISSN: 2320-7078 P-ISSN: 2349-6800 www.entomoljournal.com JEZS 2020; SP-8(2): 133-137 © 2020 JEZS Received: 12-01-2020 Accepted: 14-02-2020 C Majhi M.V.Sc. scholar, Department of Veterinary Clinical Medicine, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology CVSc. & AH, OUAT, Bhubaneswar, Odisha, India GR Jena Assistant professor, Department of Veterinary Clinical Medicine, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology (CVSc&AH, OUAT), Bhubaneswar, Odisha, India. L Das Scientist, (Animal Science), Krishi Vigyan Kendra, Keonjhar, Odisha University of Agriculture and Technology (CVSc&AH, OUAT), Bhubaneswar, Odisha, India. D Kumar Scientist, Regional centre, ICAR- Central Avian Research Institute, Bareilly, Uttar Pradesh, India SK Mishra Scientist, Regional centre, ICAR- Central Avian Research Institute, Bareilly, Uttar Pradesh, India A Mishra VAS, Department of F & ARD, Govt. of Odisha, Odisha, India MA Elmorsy Ph. D scholar, Department of Veterinary Clinical Medicine, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology CVSc and AH, OUAT, Bhubaneswar, Odisha, India MR Das Professor and head of Department of Veterinary Clinical Medicine, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology CVSc and AH, OUAT, Bhubaneswar, Odisha, India Corresponding Author: C Majhi M.V.Sc. scholar, Department of Veterinary Clinical Medicine, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology CVSc. & AH, OUAT, Bhubaneswar, Odisha, India Isolation and identification of Riemerella anatipestifer from Duck in Odisha, and its susceptibility to antibiotics and therapeutic management C Majhi, GR Jena, L Dash, D Kumar, SK Mishra, A Mishra, MA Elmorsy and MR Das Abstract This study was conducted in the Department of Veterinary Clinical Medicine, C.V.Sc & A.H, OUAT, Bhubaneswar, Odisha, India, for isolation and identification of Riemerella anatipestifer from duck in Odisha by culturing, staining and biochemical methods. Also, detection of its susceptibility to antibiotics with reference to therapeutic management and preventive vaccination were done. One hundred and twenty-six samples were collected from twenty-two different farms in Odisha. Results showed that eleven out of twenty-two susceptible farms were positive. The in-vitro antibiotic sensitivity of the organism showed high sensitivity to Ciprofloxacin, Norfloxacin, Gentamycin, Chloramphenicol and Polymyxin-B, moderate sensitivity to Doxycycline, and resistance to penicillin-G, Metronidazole, Sulphadiazine, Methicillin, Ampicillin, Cefuroxime and Erythromycin. Chloramphenicol application at therapeutic doses either through feed or water with supportive therapy like immune modulator multivitamin and hepatic stimulants reduced the mortality rate. As a preventive measure, ring vaccination strategy was adopted and gave good results in prevention of further spread of the disease. Keywords: Riemerella anatipestifer, stain, culture, Antibiotic, sensitivity, biochemical, chloramphenicol, hepatoprotective, vaccination 1. Introduction Duck rising is a lucrative livestock industry in the globe because of its egg, meat and feather. Like chicken, ducks are reared for eggs and meat. There are over 10 million duck population exists in our India and ranks the 2 nd in the world after Indonesia. In India, Kerala, West- Bengal, Odisha and Andhra Pradesh are the States where ducks are predominant. The availability of vast river beds and enormous water bodies are contributing large scope in the development of duck industry. Infectious diseases like Duck plague (Duck Viral Enteritis), Duck Cholera (Pasteurellosis) and Duck Viral Hepatitis are commonly encountered in village flocks. Most of the farmers ranked duck plague or duck viral enteritis as the most important disease, followed by duck cholera, duck septicemia, botulism and duck viral hepatitis [1] . Surveillance and monitoring programmes are the first steps towards providing an appropriate level of understanding of the health status of duck population. Hence, disease surveillance of duck population is urgently needed to detect the presence of infectious and non-infectious causative agents and to swiftly adopt counter measures to improve the productivity of ducks to assess the importance of common duck diseases and the level of control practices. Pasteurellosis (Duck cholera) caused high mortality in both domestic and wild water fowl in India [2] . Duck cholera occurs as a relatively common disease, recorded in worldwide including India, and outbreaks occur mainly in young and adult birds with 5 to 100% morbidity and 0.5 to 80% mortality rates. Economic loss to the duck industry from this disease is due to mortality, with rates ranging from 5% to 75%, as well as weight loss and condemnations. Typically, ducklings of 1 to 8-weeks old are highly susceptible. Ducklings under 5-weeks old usually die within 1 to 2 days after appearance of clinical signs, but older birds may survive longer. Stress factors such as concomitant disease or adverse environmental conditions predispose to outbreaks of the disease in ducklings. Riemerella anatipestifer is important in veterinary medicine, as it is reported worldwide as the cause of epizootic infectious