The new hyperspectral microscopic system for cancer diagnosis Yao-Fang Hsieh *1 , Ou-Yang Mang 2 , Jin-Chern Chiou 2 , Yung-Jiun Lin 2 , Ming-Hsui Tsai 3 , Da-Tian Bau 3 , Chang-Fang Chiu 3 , Guan-Chin Teseng 3 , Nai-Wen Chang 3 , Wen-Chung Kao 4 , Shun-De Wu 5 1 Dept. of Optics and Photonics, National Central Univ., Jhongli City, Taoyuan County , Taiwan 2 Dept. of Electrical Engineering, National Chiao-Tung Univ., Hsihchu city, Taiwan 3 School of Medicine, China Medical Univ., Taichung City, Taiwan 4 Dept. of Applied Electronics Technology, Taiwan Normal Univ., Taipei City, Taiwan 5 Dept. of Mechatronic Technology, Taiwan Normal Univ., Taipei City, Taiwan ABSTRACT Until now, the cancer was examined by diagnosing the pathological changes of tumor. If the examination of cancer can diagnose the tumor before the cell occur the pathological changes, the cure rate of cancer will increase. This research develops a human-machine interface for hyper-spectral microscope. The hyper-spectral microscope can scan the specific area of cell and records the data of spectrum and intensity. These data is helpful to diagnose tumor. This research aims to develop a new system and a human-machine interface to control the hyper-spectral microscope. The interface can control the moving speed of motor, the exposure-time of hyper-spectrum, real-time focus, image of fluorescence, and record the data of spectral intensity and position. Keywords: hyperspectral image, fluorescent image, cancer, diagnosis 1. INTRODUCTION As the Willoughby et al. defined, the hyperspectral image had tens or hundreds of spectral band and the order of spectral resolution was 0.01 [1]. The hyperspectral image is an image which contains the spatial and spectral information (x, y and λ). In this study, the y axis is parallel to the slit direction, x axis is the scanning direction and the λ axis is the wavelength axis. The hyperspectral image provides the spectrum of each pixel. The information of hyperspectral-image is stored as 3D data set. Also, the hyperspectral image can regard as the collection of many images which are measured at different wavelength individually. The hyperspectral image can apply to macroscopic-technical examination and documentation of objects of artistic and historic analysis and also can apply to analyze biomedical fluorescence image [2-3]. Up to now there are four scanning methods for hyper-spectral image [4] which are wavelength-scan, spatial-scan, time-scan and compromise-scan. In this article, the novel hyper-spectroscopic imaging system uses the spatial-scan method. Resulting from a complex scanning mechanism of the relative movement, we propose that an internal relay lenses would scan on the extra image plane to achieve a hyperspectral images with a better image quality. In recent years, the biomedical diagnostic technique utilizes the fluorescent spectrum to diagnosis the cancer cell. The technique utilizes the difference of fluorescent spectrum of photosensitizer in the human body to diagnose the pathological cell. As the Fig.1 Shows, the normal oral cell and the abnormal oral has the different fluorescent spectrum at red band (600nm). Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, edited by Daniel L. Farkas, Dan V. Nicolau, Robert C. Leif, Proc. of SPIE Vol. 7902, 79020J · © 2011 SPIE · CCC code: 1605-7422/11/$18 · doi: 10.1117/12.874843 Proc. of SPIE Vol. 7902 79020J-1 Downloaded From: http://proceedings.spiedigitallibrary.org/ on 01/26/2016 Terms of Use: http://spiedigitallibrary.org/ss/TermsOfUse.aspx