Int.J.Curr.Microbiol.App.Sci (2016) 5(8): 538-544 538 Original Research Article http://dx.doi.org/10.20546/ijcmas.2016.508.058 Anti-Vibrio Activity of Parkia javanica: Studies on MIC, MBC, Growth Curve Analysis and ROS Generation on Four Vibrio cholarae Strains Susmita Saha 1 , Jhinuk Basu Mullick 1 , Parichita Ray Choudhury 1 , Partha Saha 1 , Debaprasad Chakraborty 2 and Samir Kumar Sil 1 * 1 Molecular Genetics and Cell Physiology Lab., Department of Human Physiology, Tripura University, Suryamaninagar, West Tripura-799022, India 2 Department of Pathology, Tripura Medical College, Hapania, West Tripura-799014, India *Corresponding author ABSTRACT Introduction Vibrio cholerae, a Gram-negative, facultative anaerobic, comma shaped bacterium, is the causative agent of life threatening diarrheal disease called cholera (Pacini, 1854). In developing countries, infectious diseases are the important factors for morbidity and mortality in common people (Mattana et al., 2012). Parkia javanica is a very large tree and has found traditional use as food and ethnomedicine in North- East India (Maiti, 2004). It is generally used by tribal population of North- East India to cure stomach ache, dysentery, food poisoning (Majumder et al., 2009). Saha et al., 2007, reported that, Parkia javanica has antibacterial activities against both standard Gram positive and Gram negative bacterial strains. But there is no report, on anti-vibrio activity of Parkia javanica against Vibrio cholerae strains. Therefore, this work has been undertaken to explore the anti-vibrio activities of P. javanica using crude methanol extract. Materials and Methods Preparation Plant Extract Fresh stem barks of Parkia javanica was collected from Suryamaninagar, Tripura, International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 5 Number 8 (2016) pp. 538-544 Journal homepage: http://www.ijcmas.com Parkia javanica, a medicinal plant, of North-East India, was screened for anti cholera activity against 4 Vibrio cholarae strains. Fresh stem barks of Parkia javanica was collected and crude methanol extract of Parkia javanica (MEPJ) was prepared by Hahnshin rotary evaporator. The minimum inhibitory concentration of MEPJ was evaluated on Vibrio cholarae strains by serial dilution technique. 10mg/ml of MEPJ is the IC 100 dose for Vibrio cholarae strains. MEPJ also showed bactericidal activity against all four Vibrio cholarae at the same concentration. The lag phase become extended after treatment with IC 50 dose of MEPJ. Intracellular reactive oxygen species (ROS) was increased (40% - 60%) in presence of MEPJ indicating ROS mediated mechanism of growth inhibition. Keywords Parkia javanica, Vibrio cholerae, growth curve, ROS. Accepted: 23 July 2016 Available Online: 10 August 2016 Article Info