Profile of Antigen Excretory-Secretory Schistosoma Japonicum in the Development of Elisa Method to Detect Schistosomiasis in Indonesia Samarang 1 , Made Agus Nurjana 1 , Malonda Maksud 1 , Sri Murtini 2 , Fadjar Satrija 2 1 Donggala Health Research and Development Center, Donggala District Central Sulawesi 94352, Indonesia 2 Bogor Agricultural University, Bogor, Indonesia * Corresponding author. Email: samarangp@gmail.com ABSTRACT The development of ELISA method to detect patients with schistosomiasis in Indonesia is a detection of the antigens that are derived from the peripheral vessels in the blood serum. This research is conducted in the Napu Plateau of Poso Regency, Central Sulawesi, Indonesia. The goal is to develop ELISA method with polyclonal antibodies to detect antigenic-excretory antigen of S. japonicum in patients with schistosomiasis. Activities in the study include activities in the field namely the isolation of worm S. japonicum and activities in laboratories such as production, characterization and purification by Bradford methods as well as electrophoresis test to see the protein Antigen profile. The results of the study of the 30 worm Schistosoma collected from 5 rats suffered schistosomiasis, obtained as much as 42.5 ml with a protein concentration of 1.351 mg/ml, and had two patterns of polypeptide tape with a molecular weight range of 20 and 39 Dalton kilo (kDa). The conclusion of the resulting Antigen ES S. japonicum profile can induce a good polyclonal antibody in the development of ELISA method to detect the AgES in the serum of schistosomiasis. Keywords: Antigen Excretory-Secretory, Schistosoma japonicum, ELISA 1. INTRODUCTION Schistosomiasis is one of the neglected tropical diseases [NTD] endemic in 78 countries and infects more than 229 million peoples in tropical and subtropical regions [1]. There are 2 major forms of schistosomiasis, intestinal and urogenital, caused by 5 main species of blood fluke. The three main species causing human disease are Schistosoma japonicum, Schistosoma mansoni and Schistosoma haematobium [2]. Among them, S. japonicum is prevalent mainly in China, the Philippines and some areas of Indonesia [3]. Other research says there are seven species such as schistosomiasis in humans caused by Schistosoma mansoni, S. haematobium, S. japonicum, S. intercalatum, S. mekongi, S. malayensis, and S. guineensis [4]. Schistosomiasis ranks second next to malaria from parasitic infection in terms of socioeconomic and health impact in tropics and subtropics [5]. Schistosome parasites lay up to a thousand eggs per day inside the veins of their mammalian hosts. The immature eggs deposited by females against endothelia of venules will embryonate within days. Approximately 30% of the eggs will migrate to the lumen of the intestine japonicum continue the parasite life-cycle. Many eggs, however, are trapped in the liver and intestine causing the main pathology associated with Schistosoma mansoni and japonicum, the liver granulomatous response [6]. In Indonesia, schistosomiasis on humans and animals are caused by worm species Schistosoma japonicum, and only found in Central Sulawesi in Napu, Bada, and Lindu highlands [7] with an intermediary host Oncomelania hupensis lindoensis (O. hupensis lindoensis) [8]. Control program implemented Until now, we haven't been able to press numbers schistosomiasis infection, due to reinfection from various reservoirs including rats, livestock, wild animals, and even the people themselves as source of infection [9]. The development of immunological and molecular techniques is a new alternative in early diagnosis of various pathogens [10]. Molecular Xenomonitoring is a DNA-based method that has been developed to monitor the transmission of several vector-borne diseases, Advances in Biological Sciences Research, volume 12 Proceedings of the 2nd International Conference on Veterinary, Animal, and Environmental Sciences (ICVAES 2020) Copyright © 2021 The Authors. Published by Atlantis Press B.V. This is an open access article distributed under the CC BY-NC 4.0 license -http://creativecommons.org/licenses/by-nc/4.0/. 20