  Citation: Wang, P.; Wang, S.;Chen, B.; Amir, M.; Wang, L.; Chen, J.; Ma, L.; Wang, X.; Liu, Y.; Zhu, K. Light and Water Conditions Co-Regulated Stomata and Leaf Relative Uptake Rate (LRU) during Photosynthesis and COS Assimilation: A Meta-Analysis. Sustainability 2022, 14, 2840. https://doi.org/10.3390/ su14052840 Academic Editor: Antonio Caggiano Received: 27 January 2022 Accepted: 22 February 2022 Published: 28 February 2022 Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affil- iations. Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). sustainability Article Light and Water Conditions Co-Regulated Stomata and Leaf Relative Uptake Rate (LRU) during Photosynthesis and COS Assimilation: A Meta-Analysis Pengyuan Wang 1,2 , Shaoqiang Wang 1,2,3, *, Bin Chen 1,2 , Muhammad Amir 1,2 , Lei Wang 4 , Jinghua Chen 1,2 , Li Ma 1,2 , Xiaobo Wang 1,2 , Yuanyuan Liu 1,2 and Kai Zhu 1,2 1 Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences (CAS), Beijing 100101, China; wangpy.19b@igsnrr.ac.cn (P.W.); chenbin@igsnrr.ac.cn (B.C.); mamir2019@igsnrr.ac.cn (M.A.); chenjh.14b@igsnrr.ac.cn (J.C.); mali.16b@igsnrr.ac.cn (L.M.); wxbwxb1995@163.com (X.W.); liuyuanyuan182@mails.ucas.edu.cn (Y.L.); zhuk.20b@igsnrr.ac.cn (K.Z.) 2 School of Resources and Environment, University of Chinese Academy of Sciences (UCAS), Beijing 100049, China 3 School of Geography and Information Engineering, China University of Geosciences (Wuhan), Wuhan 430074, China 4 Hefei Institute of Physical Science, Chinese Academy of Sciences (CAS), Hefei 230031, China; wanglei@aiofm.ac.cn * Correspondence: sqwang@igsnrr.ac.cn Abstract: As a trace gas involved in hydration during plant photosynthesis, carbonyl sulfide (COS) and its leaf relative uptake rate (LRU) is used to reduce the uncertainties in simulations of gross pri- mary productivity (GPP). In this study, 101 independent observations were collected from 22 studies. We extracted the LRU, stomatal conductance (g s ), canopy COS and carbon dioxide (CO 2 ) fluxes, and relevant environmental conditions (i.e., light, temperature, and humidity), as well as the atmospheric COS and CO 2 concentrations (C a,COS and C a,CO2 ). Although no evidence was found showing that g s regulates LRU, they responded in opposite ways to diurnal variations of environmental conditions in both mixed forests (LRU: Hedges’d = 0.901, LnRR = 0.189; g s : Hedges’d = 0.785, LnRR = 0.739) and croplands dominated by C3 plants (Hedges’d = 0.491, LnRR = 0.371; g s : Hedges’d = 1.066, LnRR = 0.322). In this process, the stomata play an important role in COS assimilation (R 2 = 0.340, p = 0.020) and further influence the interrelationship of COS and CO 2 fluxes (R 2 = 0.650, p = 0.000). Slight increases in light intensity (R 2 = 1, p = 0.002) and atmospheric drought (R 2 = 0.885, p = 0.005) also decreased the LRU. The LRU saturation points of C a,COS and C a,CO 2 were observed when ΔC a,COS 13 ppt (R 2 = 0.580, p = 0.050) or ΔC a,CO 2 ≈−18 ppm (R 2 = 0.970, p = 0.003). This study concluded that during plant photosynthesis and COS assimilation, light and water conditions co- regulated the stomata and LRU. Keywords: meta-analysis; carbonyl sulfide (COS); carbon dioxide (CO 2 ); leaf relative uptake rates (LRU); stomatal conductance; environmental conditions 1. Introduction 1.1. Global Change and the Terrestrial Carbon Fixation Global industrialization has caused the enrichment of atmospheric greenhouse gases (GHGs) globally [1]. In the atmosphere, GHGs have changed the process of radiation transmission [2]; further, more radiation is being trapped in the atmosphere [3,4]. The long-lived GHGs have dominated 47% of global warming, in particular, CO 2 takes up 80% of this increase alone [5]. In this context, terrestrial and oceanic carbon sinks have been found to increase continuously over the last 60 years [69]. The terrestrial ecosystem responds to climate variability and climate change [1012]. Global warming and land-cover Sustainability 2022, 14, 2840. https://doi.org/10.3390/su14052840 https://www.mdpi.com/journal/sustainability