Research Article Escherichia coli O157:H7fromFoodofAnimalOrigininArsi: OccurrenceatCateringEstablishmentsandAntimicrobial SusceptibilityProfile MindaAsfawGeresu 1 andShimelisRegassa 2 1 Department of Veterinary Science, College of Agriculture and Environmental Science, Arsi University, Asella, Ethiopia 2 Department of Animal Science, College of Agriculture and Environmental Science, Arsi University, Asella, Ethiopia Correspondence should be addressed to Minda Asfaw Geresu; minda.asfaw@gmail.com Received 2 January 2021; Revised 10 March 2021; Accepted 17 March 2021; Published 29 March 2021 Academic Editor: Ahmad Mansour Copyright © 2021 Minda Asfaw Geresu and Shimelis Regassa. is is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Escherichia coli O157:H7(E. coli O157 : H7) has been found to be the major cause of food-borne diseases and a serious public health problem in the world, with an increasing concern for the emergence and spread of antimicrobial-resistant strains. Hitherto, little is known about the carriage of E. coli O157 : H7 and its antimicrobial susceptibility profile in the food of animal origin in Ethiopia. is study aimed to determine the occurrence and multidrug resistance profile of E. coli O157 : H7 from food of animal origin at different catering establishments in the selected study settings of Arsi Zone. One hundred ninety-two animal origin food items, namely, raw/minced meat (locally known as “Kitfo,” “Kurt,” and “Dulet”), raw milk, egg sandwich, and cream cake samples were collected and processed for microbiological detection of E. coli O157 :H7. Out of 192 samples, 2.1% (4/192) were positive for E. coli O157:H7.Two E. coli O157 :H7 isolates were obtained from “Dulet” (6.3%) followed by “Kurt” (3.1%, 1/32) and raw milk (3.1%, 1/32), whereas no isolate was obtained from “Kitfo,” egg sandwich, and cream cake samples. Of the 4 E. coli O157:H7 isolates subjected to 10 panels of antimicrobial discs, 3 (75%) were highly resistant to kanamycin, streptomycin, and nitro- furantoin. Besides, all the isolates displayed multidrug resistance phenotypes, 3 to 5 antimicrobial resistance, amid kanamycin, streptomycin, nitrofurantoin, tetracycline, and chloramphenicol. e occurrence of multidrug-resistant E. coli O157:H7 isolates from foods of animal origin sampled from different catering establishments reveals that the general sanitary condition of the catering establishments, utensils used, and personnel hygienic practices did not comply with the recommended standards. us, this finding calls for urgent attention toward appropriate controls and good hygienic practices in different catering establishments dealing with consuming raw/undercooked foods of animal origin. 1.Introduction Food-borne pathogens are the leading causes of food-borne human illness and death in the world [1]. e severity is higher among developing countries, including Ethiopia [2, 3]. is could be attributed to changes in eating habits, mass catering, complex and lengthy food supply procedures with increased international movement, poor food handling and sanitation practices, inadequate food safety laws, weak regulatory systems, lack of financial resources, and aware- ness about proper food handling which creates a conducive environment for the spread of food-borne and food poisoning etiologic agents [4–6]. A wide range of pathogens plays a role in food-borne disease, most of which have a zoonotic origin and have carriers in healthy food animals from which they spread to an increasing variety of foods of animal origin and are considered as major vehicles of food- borne infections [7, 8]. Among the bacterial pathogens, Escherichia coli O157:H7(E. coli O157 : H7) has frequently been associated with food-borne illness [9]. e infection caused by these bacteria initially causes nonbleeding diar- rhea accompanied by abdominal cramps. en, it may de- velop into bloody diarrhea and hemolytic uremic syndrome (HUS), which causes kidney failure in humans [10–12]. Hindawi e Scientific World Journal Volume 2021, Article ID 6631860, 10 pages https://doi.org/10.1155/2021/6631860